摘要
利用高压糊化-普鲁兰酶工艺制备黑苦荞抗性淀粉,采用响应面法优化黑苦荞抗性淀粉制备条件。在单因素实验基础上选取因素和水平,以高压糊化时间、酶解时间和酶添加量为影响因子,采用中心组合法进行3因素3水平实验设计,建立了制备工艺的二次多项数学模型,并验证了该模型的有效性,得到了最优工艺参数:高压糊化时间23 min、酶解时间4.43 h、酶添加量14 ASPU/g,得到黑苦荞抗性淀粉得率为(32.36±0.24)%,基本符合理论预测值(32.35%)。
The black Tartary buckwheat resistant starch was prepared using an autoclave-pullulanase process,and the response surface methodology was used to optimize the resistant starch preparation conditions.Based on the single-factor test,a 3-factor,3-level experimental design was conducted by using the central group method with autoclaving time,enzymatic digestion time and enzyme addition as the influencing factors,and a quadratic multinomial mathematical model was established for the preparation process,as well as the validity of the model was verified.The optimal process parameters were obtained:autoclaving time of 23 min,enzymatic digestion time of 4.43 h,and enzyme addition of 14 ASPU/g.The yield of resistant starch of black Tartary buckwheat was(32.36±0.24)%,basically consistent with the predicted value of the model(32.35%).
作者
郑发英
赵梓娴
曾姗姗
彭晓宇
刘思曼
涂平
邢亚阁
许青莲
陈剑雄
张萍
Zheng Faying;Zhao Zixian;Zeng Shanshan;Peng Xiaoyu;Liu Siman;Tu Ping;Xing Yage;Xu Qinglian;Chen Jianxiong;Zhang Ping(Xihua University School of Food and Bioengineering ,Chengdu 611743;Huantai Biotechnology Co.,Ltd.,Chengdu 610200)
出处
《中国粮油学报》
CAS
CSCD
北大核心
2023年第12期99-105,共7页
Journal of the Chinese Cereals and Oils Association
基金
四川省科技计划项目(2018NZ0097),成都市科技局项目(2022-YF05-00914-SN)。
关键词
响应面法
黑苦荞
抗性淀粉
普鲁兰酶
高压糊化
response surface methodologyt
black tartary buckwheat
resistant starch
pullulanase
autoclave