冠心病患者血清lncRNA H19、CHAST的表达水平及临床意义

Expression and Clinical Significance of Serum lncRNA H19,CHAST in Patients with Coronary Heart Disease

目的探讨冠心病(coronary heart disease,CHD)患者血清长链非编码RNA(lncRNA)H19、CHAST的表达水平及临床意义。方法选取2021年6月~2022年5月在笔者科室住院的冠心病患者135例,其中急性冠状动脉综合征76例,慢性冠状动脉综合征59例,选取同期住院且经冠状动脉造影排除CHD的对照组62例。采用实时荧光定量PCR(RT-PCR)法测量各组血清lncRNA H19、lncRNA CHAST的表达水平,同时检测各组血清超敏C反应蛋白(high sensitivity C-reactive protein,hs-CRP)、肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)、白细胞介素(interleukin,IL)-6、低密度脂蛋白(low density lipoprotein,LDL)、高密度脂蛋白(high density lipoprotein,HDL)等指标。采用ROC曲线分析血清lncRNA H19、lncRNA CHAST表达水平对CHD的诊断价值;Pearson相关性分析评估lncRNA H19、lncRNA CHAST表达水平与hs-CRP、TNF-α、IL-6、LDL、HDL等因素的相关性;Logistics回归分析CHD的危险因素。结果CHD组血清lncRNA H19、lncRNA CHAST表达水平较对照组升高(P<0.05)。CHD组hs-CRP、IL-6、TNF-α、LDL水平高于对照组(P<0.05),HDL水平低于对照组(P<0.05),lncRNA H19、lncRNA CHAST表达水平与hs-CRP、IL-6、TNF-α、LDL等指标呈正相关(P<0.05),与HDL呈负相关(P<0.05)。lncRNA H19、lncRNA CHAST为CHD的危险因素,且两者能较好的诊断CHD。结论lncRNA H19、lncRNA CHAST为CHD发病的危险因素,可作为诊断CHD的血清指标,且二者联合诊断CHD的价值更高。

Objective To investigate the expression level and clinical significance of serum long noncoding RNA(lncRNA)H19,CHAST in patients with coronary heart disease(CHD).Methods A total of 135 patients with CHD who were admitted to the Department of Cardiovascular Medicine of Lianyungang Second People′s Hospital from June 2021 to May 2022 were selected,including 76 cases of acute coronary syndrome and 59 cases of chronic coronary syndrome.We selected 62 patients in the control group who were hospitalized and excluded from CHD by coronary angiography.Real-time fluorescent quantitative PCR(RT-PCR)was used to detect the relative expression level of lncRNA H19 and lncRNA CHAST in the circulating serum of the subjects.Meanwhile,the high sensitivity C-reactive protein(hs-CRP)、tumor necrosis factor-α(TNF-α)、interleukin-6(IL-6)、low density lipoprotein(LDL)、high density lipoprotein(HDL)and other related indicators of each group were collected.The receiver operating characteristic(ROC)curve was used to analyze the clinical diagnostic value of circulating serum lncRNA H19 and lncRNA CHAST expression level in the diagnosis of CHD.Pearson correlation coefficient was used to analyze the correlation between serum lncRNA H19,lncRNA CHAST level,and other related indicators.Multivariate Logistic regression was used to analyze the risk factors of CHD.Results The serum expression levels of lncRNA H19 and lncRNA CHAST in the CHD group were higher than those in the control group(P<0.05).The serum levels of hs-CRP、TNF-α、IL-6、and LDL in the experimental group were higher than those in the control group(P<0.05),and the serum levels of HDL were lower than those in the control group(P<0.05).The expression levels of lncRNA H19 and lncRNA CHAST were positively correlated with hs-CRP,IL-6 and LDL,and were negatively correlated with HDL.lncRNA H19 and lncRNA CHAST are risk factors for CHD,which may have a higher value in the diagnosis of CHD.Conclusion lncRNA H19 and lncRNA CHAST are risk factors for CHD and can be used as serum indicators for the diagnosis of CHD,and the combination of the two has a higher value in the diagnosis of CHD.In addition,the expression levels of both were correlated with the levels of inflammatory factors and apolipoproteins.