hsa_circ_401724表达与2型糖尿病患者的炎症反应及与胰岛细胞功能的关系研究

Relationship between hsa_circ_401724 expression and inflammatory response and pancreatic islet cell function in type 2 diabetic patients

目的分析hsa_circ_401724表达与2型糖尿病(T2DM)患者的炎症反应及与胰岛细胞功能的关系。方法选取2017年4月至2022年12月临汾市中心医院收治的102例T2DM患者作为观察组,同期选取该院100例糖耐量正常的体检健康者作为对照组。采用酶联免疫吸附试验法检测受试者血中肿瘤坏死因子α(TNF-α)、白细胞介素6(IL-6)及细胞间黏附分子-1(ICAM-1)水平评估受试者炎症因子水平。依照检测熔解曲线计算hsa_circ_401724/U6相对表达水平,以及评估受试者胰岛细胞功能,包括胰岛素抵抗指数(HOMA-IR)、稳态模型评估的胰岛β细胞功能指数(HOMA-β)水平。Pearson相关性分析hsa_circ_401724表达水平与炎症反应、胰岛细胞功能的相关性,采用Logistic回归模型分析hsa_circ_401724表达水平与炎症反应、胰岛细胞功能的关系。结果观察组患者HOMA-IR、TNF-α、IL-6、ICAM-1水平明显高于对照组,观察组患者HOMA-β水平明显低于对照组,差异有统计学意义(P<0.05)。观察组患者hsa_circ_401724相对表达水平(0.75±0.13)明显高于对照组(0.24±0.06),差异有统计学意义(P<0.05)。hsa_circ_401724高表达组患者HOMA-IR、TNF-α、IL-6、ICAM-1水平明显高于hsa_circ_401724低表达组,且HOMA-β水平明显低于hsa_circ_401724低表达组,差异有统计学意义(P<0.05)。hsa_circ_401724相对表达水平与HOMA-IR、TNF-α、IL-6、ICAM-1水平呈正相关(r=0.657、0.671、0.703、0.698,P<0.05),hsa_circ_401724表达水平与HOMA-β水平呈负相关(r=-0.611,P<0.05)。hsa_circ_401724高表达是影响T2DM患者HOMA-IR、HOMA-β、TNF-α、IL-6、ICAM-1水平的独立危险因素(P<0.05)。结论hsa_circ_401724高表达与T2DM患者的炎症反应及其胰岛细胞功能下降有关。

Objective To analyze the relationship between the expression of hsa_circ_401724 and the inflammatory response in type 2 diabetes mellitus(T2DM)patients and pancreatic islet cell function.Methods A total of 102 patients with T2DM treated in Linfen Central Hospital from April 2017 to December 2022 were selected as the observation group,and 100 healthy subjects with normal glucose tolerance were selected as the control group during the same period.The levels of tumor necrosis factorα(TNF-α),interleukin-6(IL-6)and intercellular adhesion molecule-1(ICAM-1)in the blood of the subjects were detected by enzyme-linked immunosorbent assay to evaluate the levels of inflammatory factors in the subjects.The relative expression level of hsa_circ_401724/U6 was calculated according to the dissolution curve,and the pancreatic islet cell function of the subjects was assessed,including homeostasis model assessment of insulin resistance(HOMA-IR)and homeostatic model assessment beta cell function(HOMA-β)as assessed by homeostasis model.Pearson correlation was used to analyze the correlation between hsa_circ_401724 expression level and inflammation and pancreatic islet cell function,and Logistics regression model was used to analyze the relationship between hsa_circ_401724 expression level and inflammation and pancreatic islet cell function.Results The levels of HOMA-IR,TNF-α,IL-6 and ICAM-1 in observation group were significantly higher than those in control group,while the levels of HOMA-βin observation group were significantly lower than those in control group,with statistical significance(P<0.05).The relative expression level of hsa_circ_401724 in observation group(0.75±0.13)was significantly higher than that in control group(0.24±0.06),and the difference was statistically significant(P<0.05).The levels of HOMA-IR,TNF-α,IL-6 and ICAM-1 in hsa_circ_401724 high expression group were significantly higher than those in hsa_circ_401724 low expression group,and the levels of HOMA-βwere significantly lower than those in hsa_circ_401724 low expression group.The difference was statistically significant(P<0.05).The relative expression level of hsa_circ_401724 was positively correlated with the levels of HOMA-IR,TNF-α,IL-6 and ICAM-1(r=0.657,0.671,0.703,0.698,P<0.05).hsa_circ_401724 expression level was negatively correlated with HOMA-βlevel(r=-0.611,P<0.05).The high expression of hsa_circ_401724 was an independent risk factor affecting the levels of HOMA-IR,HOMA-β,TNF-α,IL-6 and ICAM-1 in T2DM patients(P<0.05).Conclusion The high expression of hsa_circ_401724 is related to the inflammatory response and the decline of pancreatic islet cell function in T2DM patients.