野西瓜多糖上调LINC00476对胰腺癌细胞增殖、迁移、侵袭的影响

Capparis spionosa polysaccharide affects proliferation,migration and invasion of pancreatic cancer cells by up-regulating LINC00476

目的探讨野西瓜多糖通过调控LINC00476对胰腺癌细胞增殖、迁移、侵袭的影响。方法将胰腺癌SUIT-2细胞分为空白组和低、中、高剂量实验组,分别用0、200、400、600μg·mL^(-1)的野西瓜多糖处理SUIT-2细胞。用克隆形成实验检测细胞的增殖能力,用Transwell实验检测细胞的迁移、侵袭能力,用实时荧光定量聚合酶链反应法检测LINC00476和人抗原R(HuR)mRNA的表达水平,用蛋白质印迹法检测HuR蛋白的表达水平。结果空白组和低、中、高剂量实验组的克隆形成数分别为(137.31±9.43)、(109.57±4.72)、(77.28±5.76)和(50.74±6.76)个,迁移细胞数分别为(229.21±27.67)、(188.69±9.29)、(150.98±20.25)和(109.37±8.04)个,侵袭细胞数分别为(125.96±9.20)、(100.52±7.60)、(81.65±8.62)和(66.01±5.79)个,LINC00476 mRNA表达水平分别为0.96±0.07、1.38±0.16、1.68±0.18和2.18±0.30,HuR mRNA表达水平分别为1.05±0.07、0.81±0.06、0.58±0.10和0.32±0.04,HuR蛋白相对表达水平分别为1.00±0.09、0.74±0.05、0.55±0.03和0.32±0.02。与空白组比较,低、中、高剂量实验组的增殖、迁移和侵袭能力均显著降低,HuR表达水平均显著降低,LINC00476 mRNA表达水平均显著升高(均P<0.05)。结论野西瓜多糖可能通过上调LINC00476抑制胰腺癌细胞的增殖、迁移、侵袭。

Objective To investigate the effect of Capparis spionosa polysaccharides on the proliferation,migration and invasion of pancreatic cancer cells by regulating LINC00476.Methods Pancreatic cancer cells SUIT-2 were divided into blank group and experimental-L,-M,-H groups,which were treated with 0,200,400,600μg·mL^(-1 )Capparis spionosa polysaccharides,respectively.Clone formation experiments were used to detect cell proliferation ability,Transwell experiments were used to detect cell migration and invasion ability,real-time fluorescent quantitative polymerase chain reaction was used to detect the expression of LINC00476 and human antigen R(HuR)mRNA,and Western blot was used to detect the expression levels of HuR proteins.Results The colony formation number in blank group and experimental-L,-M,-H groups were 137.31±9.43,109.57±4.72,77.28±5.76 and 50.74±6.76;the number of migrating cells were 229.21±27.67,188.69±9.29,150.98±20.25 and 109.37±8.04;the n umber of invasive cells were 125.96±9.20,100.52±7.60,81.65±8.62 and 66.01±5.79;the expression levels of LINC00476 mRNA were 0.96±0.07,1.38±0.16,1.68±0.18 and 2.18±0.30;the expression levels of HuR mRNA were 1.05±0.07,0.81±0.06,0.58±0.10 and 0.32±0.04;the relative expression levels of HuR protein were 1.00±0.09,0.74±0.05,0.55±0.03 and 0.32±0.02.Compared with blank group,the proliferation,migration and invasion abilities of experimental-L,-M,-H groups were decreased,the expression levels of HuR were decreased,and the expression levels of LINC00476 mRNA were increased,the differences were statistically significant(all P<0.05).Conclusion Capparis spionosa polysaccharides may inhibit the proliferation,migration and invasion of pancreatic cancer cells by up-regulating LINC00476.