黄芩配伍桑白皮的镇痛抗炎作用研究

Analgesic and Anti-inflammatory Effects of Scutellaria baicalensis Georgi Combined with Morus alba L.

探究黄芩配伍桑白皮的镇痛、抗炎作用。本研究采用冰醋酸诱导小鼠扭体反应,通过观察扭体次数来评价其外周性镇痛作用;采用常规热板法致小鼠疼痛并通过测定痛阈值来评价其中枢性镇痛作用;采用二甲苯致小鼠耳廓肿胀计算耳肿胀度并通过肿胀抑制率评价其局部抗炎作用;采用20%酵母悬液致大鼠全身炎症性反应并测定血清中前列腺素E2(PGE_(2))、白细胞介素-6(IL-6)和肿瘤坏死因子(TNF-α)炎症因子水平和肺组织损伤状况研究其抗炎作用。结果表明,黄芩配伍桑白皮能明显减少醋酸致小鼠的扭体次数(P<0.05,P<0.01),延长热板法致小鼠疼痛的痛阈值,能显著抑制二甲苯致小鼠耳廓肿胀并降低由20%酵母悬液建立全身炎性反应的大鼠血清中PGE_(2)、IL-6和TNF-α炎症因子水平(P<0.01),改善肺组织损伤程度,且效果优于黄芩和桑白皮单味药组。黄芩配伍桑白皮具有一定的镇痛、抗炎作用,推测作用机制可能与抑制PGE_(2)、IL-6和TNF-α的合成或释放有关。

To explore the analgesic and anti-inflammatory effects of Scutellaria baicalensis Georgi combined with Morus alba L..Peripheral analgesia was evaluated by using the glacial acetic acid to induce writhing response in mice and observing the number of writhing;The central analgesic effect was evaluated by the conventional hot plate method to measure pain threshold in mice;The local anti-inflammatory effect was evaluated by using xylene-induced ears swelling in mice to calculate the degree of ear swelling and swelling inhibition rate;the systemic inflammatory response of rats induced by 20% yeast suspension was used to determine serum prostaglandin E_(2)(PGE_(2)),interleukin-6(IL-6),and tumor necrosis factor(TNF-α) inflammatory factors level and lung tissue damage status to study its anti-inflammatory effect.The combination of S.baicalensis combined with M.alba can significantly reduce the number of writhing in mice induced by acetic acid(P <0.05,P<0.01),prolong the pain threshold of pain in mice induced by hot plate method,significantly inhibit the swelling of mouse auricles induced by xylene,and reduce the establishment of systemic inflammation by 20% yeast suspension the levels of PGE_(2),IL-6 and TNF-α in the serum of sexually reactive rats were improved(P <0.01),and the degree of lung tissue damage was improved,and the effect was better than that of S.baicalensis and M.alba.The compatibility of S.baicalensis combined with M.alba has certain analgesic and antiinflammatory effects,and the mechanism may be related to the inhibition of the synthesis or release of PGE_(2),IL-6 and TNF-α inflammatory factors.