产朊假丝酵母发酵蛋白桑的培养基优化

Optimization of Medium for Fermentation of Protein Mulberry by Candida utilis

以蛋白桑桑叶为研究对象,利用产朊假丝酵母菌进行培养料发酵试验。根据单因素试验结果,设计并实施了葡萄糖、磷酸二氢钾(KH2PO4)、硫酸铵[(NH4)_(2)SO_(4)]、硫酸镁(MgSO_(4))、蒸馏水五因素四水平的正交试验,发酵后测定了产朊假丝酵母菌的菌数,确定了最佳的培养基配方,即1.2 g葡萄糖、0.025 g KH2PO4、0.05 g(NH4)_(2)SO_(4)、0.001 g MgSO_(4)、16 mL蒸馏水。在此培养基中,产朊假丝酵母菌能以菌数多达10.05亿/g进行生长繁殖。

The protein mulberry leaf was selected as the research object,and a fermentation experiment was conducted using Candida utilis.Based on the results of a sinple factor experiment,an orthogonal experiment was designed and implemented at four levels with five factors of glucose,potassium dihydrogen phosphate(KH2PO4),ammonium sulfate(NH4)_(2)SO_(4),magnesium sulfate(MgSO_(4))and water.After fermentation,the biomass of Candida utilis was determined.The optimal medium formula was determined,which includes 1.2 g glucose,0.025 g KH2PO4,0.05 g(NH4)_(2)SO_(4),0.001 g MgSO_(4)and 16 mL distilled water.In this culture medium,Candida utilis can grow and reproduce with a maximum bacterial count of 1.005 billion/g.