木犀草素对D-半乳糖所致小鼠认知障碍的改善作用

Ameliorative effect of luteolin on D-galactose induced cognitive impairment in mice

目的木犀草素对D-半乳糖所致小鼠认知障碍的改善作用及可能机制。方法将小鼠随机分为正常组、模型组、木犀草素组,每组10只。通过水迷宫检测等行为学、免疫组织荧光法检测小鼠海马认知行为及相关蛋白的表达。结果各组间的小鼠活动度差异不显著;模型组小鼠新旧食物识别指数显著降低,小鼠找到平台的潜伏期延长,小鼠在目标象限的停留时间缩短,海马成熟神经元数目降低,突触相关蛋白表达下降;木犀草素组小鼠新旧食物识别指数显著增加,小鼠找到平台的潜伏期缩短,小鼠在目标象限的停留时间延长,海马成熟神经元数目增多,突触相关蛋白表达上调。结论木犀草素可能通过增强氧自由基清除能力,保护海马神经元,增强神经突触可塑性,从而改善D-半乳糖导致的小鼠认知障碍。

【Objective】To investigate the effect and possible mechanism of luteolin on D-galactose induced cognitive impairment in mice.【Methods】Mice were randomly divided into normal group,model group and luteolin group,with 10 mice in each group.Hippocampal cognitive behaviors and expressions of related proteins were detected by water maze detection and immunofluorescence.【Results】There was no significant difference in activity between the groups.In the model group,the old and new food recognition index was significantly decreased,the latency of finding the platform was prolonged,the residence time in the target quadrant was shortened,the number of mature neurons in the hippocampus was decreased,and the expression of synaptic related proteins was decreased.The old and new food recognition index of mice in luteolin group was significantly increased,the latency of finding the platform was shortened,the residence time of mice in the target quadrant was prolonged,the number of mature neurons in hippocampus was increased,and the expression of synaptic related proteins was up-regulated.【Conclusion】Luteolin may improve D-galactose induced cognitive impairment in mice by enhancing oxygen free radical scavenging ability,protecting hippocampal neurons and enhancing synaptic plasticity.