PCOS患者血清miR-206表达及其与下丘脑-垂体-性腺轴内分泌的关系分析

Expression of serum miR-206 in patients with PCOS and its relationship with the endocrine of hypothalamus-pituitary-gonad axis

目的探讨多囊卵巢综合征(PCOS)患者血清微小核糖核酸-206(miR-206)表达及其与下丘脑-垂体-性腺轴内分泌的关系。方法选取2020年3月至2022年12月许昌市人民医院收治的148例PCOS患者设为研究组,另同期选取133例月经正常的健康体检未孕女性设为对照组。采用实时荧光定量聚合酶链式反应(qRT-PCR)检测所有受试者血清miR-206表达,另采用化学发光免疫分析法检测血清促卵泡激素(FSH)、促黄体生成素(LH)、雌二醇(E2)、睾酮(T)、泌乳素(PRL)、孕酮(P)水平。采用Pearson相关系数分析PCOS患者血清miR-206表达与FSH、LH、E2、T、PRL、P的关系。结果研究组血清miR-206表达(0.38±0.07)明显低于对照组(0.95±0.12)(P<0.05);研究组血清FSH、P水平[(5.12±1.19)mIU/mL、(1.52±0.44)nmol/L]均明显低于对照组[(6.58±1.34)mIU/mL、(2.49±0.62)nmol/L],血清LH、E2、T、PRL水平[(10.96±2.24)mIU/mL、(78.63±22.49)pg/mL、(0.88±0.23)ng/mL、(21.46±5.32)ng/mL]均明显高于对照组[(6.73±1.85)mIU/mL、(49.41±10.65)pg/mL、(0.51±0.12)ng/mL、(16.35±3.21)ng/mL],差异有统计学意义(P<0.05);经Pearson相关系数分析,PCOS患者血清miR-206表达与LH、E2、T、PRL水平均呈负相关性(r=-0.546、-0.501、-0.533、-0.485,P<0.05),与FSH、P水平均呈正相关性(r=0.493、0.528,P<0.05)。结论PCOS患者血清miR-206表达明显降低,其与下丘脑-垂体-性腺轴内分泌相关指标存在一定的相关性。

【Objective】To investigate the expression of serum microribonucleic acid-206(miR-206)in patients with polycystic ovary syndrome(PCOS)and its relationship with hypothalamus-pituitary-gonad axis endocrine.【Methods】Totally 148 PCOS patients admitted to our hospital from March 2020 to December 2022 were selected as the study group,and 133 healthy non-pregnant women with normal menstruation were selected as the control group at the same time.Real-time fluorescence quantitative polymerase chain reaction(qRT-PCR)was used to detect the expression of miR-206 in serum of all subjects,and the serum levels of follicle-stimulating hormone(FSH),luteinizing hormone(LH),estradiol(E2),testosterone(T),prolactin(PRL)and progesterone(P)were detected by chemiluminescence immunoassay.The relationships between serum miR-206 expression and FSH,LH,E2,T,PRL,P in patients with PCOS were analyzed by Pearson correlation coefficient.【Results】The expression of serum miR-206 in the study group(0.38±0.07)was significantly lower than that in the control group(0.95±0.12)(P<0.05).The serum FSH and P levels in the study group(5.12±1.19 mIU/mL,1.52±0.44 nmol/L)were significantly lower than those in the control group(6.58±1.34 mIU/mL,2.49±0.62 nmol/L),and the levels of serum LH,E2,T and PRL(10.96±2.24 mIU/mL,78.63±22.49 pg/mL,0.88±0.23 ng/mL,21.46±5.32 ng/mL)were significantly higher than those in the control group(6.73±1.85 mIU/mL,49.41±10.65 pg/mL,0.51±0.12 ng/mL,16.35±3.21 ng/mL),and the differences were statistically significant(P<0.05).Pearson correlation coefficient analysis showed that the expression of serum miR-206 of PCOS patients was negatively correlated with the levels of LH,E2,T and PRL(r=-0.546,-0.501,-0.533,-0.485,P<0.05),but it was positively correlated with the levels of FSH and P(r=0.493,0.528,P<0.05).【Conclusion】The expression of serum miR-206 of patients with PCOS is significantly decreased,and there is a certain correlation between it and the endocrine related indexes of hypothalamus-pituitary-gonad axis.