BPQDs@TNTs药物载体的构建及其缓释性能研究

Construction of BPQDS@TNTS drug carrier and its sustained release properties

目的在骨科钛板表面构建能负载阿仑膦酸钠及硫酸庆大霉素的双载药复合药物载体。方法通过阳极氧化法在TiO_(2)表面制备纳米管阵列(TNTs),动态吸附阿伦磷酸钠后再填充载有硫酸庆大霉素的载药黑磷量子点(BPQDs),管口采用肉豆蔻醇进行封装,并利用X射线衍射、透射电镜进行表征,最后通过液相色谱法测定其上负载的阿仑膦酸钠及硫酸庆大霉素的缓释速率。结果X射线衍射表明构建的BPQDs@TNTs药物载体具备BP和TNTs的特征衍射峰,透射电镜扫描表明,肉豆蔻醇成功地对TNTs进行了封口,TiO_(2)纳米管中的阿仑膦酸钠及BPQDs载体的硫酸庆大霉素在72 h内已基本释放完毕,BPQDs@TNTs药物载体上阿仑膦酸钠及硫酸庆大霉素则在96 h内才基本释放完毕。结论BPQDs@TNTs药物载体能成功搭载阿仑膦酸钠及硫酸庆大霉素,且在体液中具有更好的缓释效能。

Objective To construct a dual-drug-loaded composite drug carrier loaded with alendronate and gentamicin on orthopaedic titanium plate.Methods Nanotube arrays(TNTs)were prepared on the surface of TiO_(2)by anodic oxidation,then dynamically adsorbed sodium alendronate and were filled with gentamicin sulfate-loaded BP quantum dots(BPQDs).The tube mouth was encapsulated with nutmeg.The tube was characterized by X-ray diffraction and transmission electron microscopy.The sustained release rate of alendronate and gentamicin loaded on the tube was determined by chromatography.Results X-ray diffraction showed that the BPQDS@TNTS drug carrier had characteristic diffraction peaks of BP and TNTs.Transmission electron microscopy showed that nutmeg had successfully sealed TNTs,the alendronate and sulphate gentamicin in TiO_(2)nanotubes and BPQDs carriers were almost completely released within 72 hours,while the alendronate and sulphate gentamicin in BPQDs@TNTs carriers were almost completely released within 96 hours.Conclusion BPQDs@TNTs drug carrier can successfully carry alendronate and gentamicin sulfate,and has better sustained-release efficacy in body fluids.