西藏羊和白-藏杂交羊外周血IFN-α、IgG和IL-2的研究

Changes in IFN-α,IgG,and IL-2 content in the peripheral blood of Tibetan sheep and White-Tibetan sheep

为了解西藏羊和白萨福克羊与西藏羊的杂交后代羊生长发育中免疫因子的分泌特点和免疫学特征,以西藏羊和白-藏杂交羊为研究对象,应用ELISA方法检测0.5、1和1.5岁外周血中干扰素(IFN-α)、免疫球蛋白G(IgG)和白细胞介素-2(IL-2)的质量浓度,分析其变化规律。结果表明:随年龄的增长,藏公羊IL-2呈逐渐降低的趋势,而藏母羊呈逐渐升高的趋势(P<0.05);藏杂交公羊和藏母羊IgG含量都呈逐渐降低趋势,藏公羊下降较明显(P<0.05);藏公羊IFN-α的含量逐渐降低,0.5岁与1岁之间下降趋势明显(P<0.05),而藏母羊变化不大。随年龄的增长,白-藏杂交羊公羊和母羊IL-2含量先降低再升高;白-藏杂交羊公羊IgG含量变化不大,母羊则是先升高后下降趋势,且1岁和1.5岁之间下降明显(P<0.05);白-藏杂交羊公羊IFN-α的含量逐渐降低,而母羊是先升高后下降。

In order to analyze the secretion characteristics of some immune molecules in the growth and development of Tibetan sheep and White-Tibetan hybrid sheep,the immunological characteristics of Tibetan sheep and White-Tibetan hybrid sheep were discussed.The concentrations of interferon(IFN-α),immunoglobulin G(IgG)and interleukin-2(IL-2)in the peripheral blood of 0.5-year-old,1-year-old and 1.5-year-old Tibetan sheep and white-Tibetan sheep were detected by ELISA,and the change rule was analyzed.The results showed that with the increase of age,IL-2 of Tibetan rams decreased gradually,while that of Tibetan ewes increased gradually(P<0.05).The IgG content of Tibetan rams and Tibetan ewes decreased gradually,and the decrease of Tibetan rams was obvious(P<0.05).The content of IFN-αin Tibetan rams gradually decreased,and the decreasing trend was obvious between 0.5 and 1 years old(P<0.05),while the change in Tibetan ewes was not significant.With the increase of age,the IL-2 content of white-Tibetan rams and ewes first decreased and then increased;the IgG content of white-Tibetan rams did not change significantly,while that of ewes increased first and then decreased,and the decrease was significant between 1 and 1.5 years old(P<0.05).The content of IFN-αin white-Tibetan rams gradually decreased,while that in ewes increased first and then decreased.