用于深层组织光学成像的荧光探针与成像技术

Fluorescent probes and imaging techniques for deep-tissue optical imaging:a review

对用于深层组织成像的探针和自适应光学成像技术的最新研究进展进行了综述,旨在为生命科学和跨学科的研究提供了一个新的思路和视角。首先,对发射光波长在近红外-Ⅱ区的荧光、生物发光、化学发光和长余辉发光的探针进行了阐述,同时,对直接波前传感方法实现快速测量和校正波畸变,以及间接波前传感方法实现校正复杂光学像差的相关技术进行了概述。上述技术和方法的不断更新,使得光学成像能够成功地穿透更深层的组织,减少背景噪声以获得更高的成像质量。

The research advancements in probes for deep-tissue imaging and adaptive optical imaging technologies are summarized,aiming to offer a new perspective for life science and interdisciplinary research.The review firstly gives an introduction on the probes emitting in the near-infrared-Ⅱ region,including fluorescence,bioluminescence,chemiluminescence,and persistent luminescence,and then elaborates direct sensing methods for rapid measurement and correction of wavefront distortions,as well as indirect sensing methods for correcting complex optical aberrations.The continuous updating of the above techniques and methods has enabled optical imaging to successfully penetrate deeper tissues with a remarkable reduction of background noise for higher image quality.