红茶对新生幼鼠肠道菌群形成的影响

The effect of black tea on the formation of intestinal flora in newborn mice

目的探讨红茶对新生幼鼠肠道菌群形成的影响,为从微生态角度研究红茶在人体肠道菌群形成过程中的作用机制奠定基础。方法利用宏基因组测序技术检测4周龄新生幼鼠(4weeks组,n=30)、12周龄正常对照组小鼠(control组,n=15)和12周龄喂饲红茶水实验组小鼠(teadrink组,n=15)的肠道菌群分布,分析3组样本的菌群差异情况,探求红茶对新生幼鼠肠道菌群形成的影响。结果与control组相比,teadrink组小鼠肠道拟杆菌门(t=-7.711,P<0.001)、拟杆菌科(t=-3.411,P=0.009)、长尾嗤菌体科(t=-2.515,P=0.036)、拟杆菌属(t=-2.693,P=0.027)、邓肯菌属(t=-2.434,P=0.041)、居海事城球杆菌属(t=-3.327,P=0.029)、迪博邓肯菌(t=-2.679,P=0.028)、普通居海事城球杆菌(t=-3.401,P=0.027)和Duncaniella_sp._C9(t=-3.104,P=0.035)相对丰度显著增加,厚壁菌门(t=8.952,P<0.001)、乳杆菌科(t=13.102,P<0.001)、消化链球菌科(t=3.665,P=0.021)、爱格菌科(t=4.481,P=0.002)、理研菌科(t=3.626,P=0.022)、乳杆菌属(t=5.542,P=0.004)、黏液乳杆菌属(t=6.334,P=0.002)、龙包茨菌属(t=3.785,P=0.005)、阿德勒菌属(t=4.504,P=0.002)、约氏乳杆菌(t=4.282,P=0.011)和罗伊氏粘液乳杆菌(t=6.156,P=0.003)相对丰度显著减少。与4weeks组相比,teadrink组小鼠肠道菌群分布差异大于control组。结论红茶对新生幼鼠肠道菌群的形成能够产生影响,不同丰度的菌群可能通过调节碳水化合物代谢等途径来达到改善肠道菌群结构、增强肠道稳态的目的,具体代谢机制有待深入研究。

Objective To explore the effect of black tea on the formation of intestinal flora in newborn mice, and lay the foundation for studying the effect of black tea in the formation of human intestinal flora from microecological perspective. Methods The intestinal flora of four-week-old neonatal group (n = 30), twelve-week-old normal control group (n = 15) and twelve-week-old tea-fed observation group (n = 15) were analyzed by using macro-genome sequencing technology to compare and analyze the differences in the flora among the three groups and to clarify the effect of black tea on the formation of intestinal flora in neonatal mice. Results Compared with the control group, the relative abundance of Bacteroidetes (t = −7.711, P<0.001), Bacteroidaceae (t = −3.411, P = 0.009), Siphoviridae (t = −2.515, P = 0.036), Bacteroides (t = −2.693, P = 0.027), Duncaniella (t = −2.434, P = 0.041), Phocaeicola (t = −3.327, P = 0.029), Duncaniella dubosii (t = −2.679, P = 0.028), Phocaeicola vulgatus (t = −3.401, P = 0.027) and Duncaniella_sp._C9 (t = −3.104, P = 0.035) in the tea-fed group significantly increased, while that of Firmicutes (t = 8.952, P<0.001), Lactobacillaceae (t = 13.102, P<0.001), Peptostreptococcaceae (t = 3.665, P = 0.021), Eggerthellaceae (t = 4.481, P = 0.002), Rikenellaceae (t = 3.626, P = 0.022), Lactobacillus (t = 5.542, P = 0.004), Limosilactobacillus (t = 6.334, P = 0.002), Romboutsia (t = 3.785, P = 0.005), Adlercreutzia (t = 4.504, P = 0.002), Lactobacillus johnsonii (t = 4.282, P = 0.011) and Limosilactobacillus reuteri (t = 6.156, P = 0.003) significantly reduced. Compared with the 4-weeks group, the difference in flora distribution in the tea-fed group was greater than that in the control group. Conclusion Black tea has an impact on the formation of gut microbiota in newborn mice. Different abundances of microbiota may achieve the goal of improving gut microbiota structure and enhancing intestinal homeostasis by regulating carbohydrate metabolism and other pathways. The specific metabolic mechanism needs to be further studied.