天然朱砂与合成朱砂的催眠药效及急性毒性比较研究

Comparative Study on Hypnotic Efficacy and Acute Toxicity of Natural and Synthetic Cinnabar

目的:比较天然朱砂与合成朱砂的药效及急性毒性差异。方法:采用水合氯醛阈上催眠实验模型,以小鼠睡眠潜伏期及睡眠时长为指标,考察两种朱砂的催眠作用药效是否存在差异;以最大给药浓度和最大给药体积(48 g/kg)进行灌胃给药,观察两种朱砂的小鼠急性毒性反应;以小鼠体质量、小鼠脏器指数为指标,并观察小鼠不同脏器组织病理切片,观察两种朱砂对不同脏器毒性的影响。结果:水合氯醛催眠实验模型显示,不同剂量的天然朱砂与合成朱砂(16.67、83.33 mg/kg)均可延长水合氯醛诱导的小鼠睡眠时长,表明两种朱砂均具有催眠作用。在一次性灌胃600倍临床用药最大剂量(48 g/kg)情况下,两种朱砂均对小鼠无急性毒性作用,小鼠体质量、小鼠脏器指数与空白组比较无显著性差异,对各脏器均未产生明显毒性。结论:天然朱砂与合成朱砂均具有催眠作用,药效无显著性差异,且均无明显急性毒性作用。

Objective:To compare the efficacy and toxicity of natural cinnabar and synthetic cinnabar.Methods:This paper adopted the suprathreshold hypnosis experiment model of chloral hydrate,and used the sleep latency and sleep duration of mice as indicators to investigate whether there is a difference in the hypnotic efficacy of the two types of cinnabar.The acute toxicity of two types of cinnabar in mice was observed after being gavaged at the maximum concentration and maximum volume of the drug(48 g/kg).The effects of two kinds of cinnabar on the toxicity of different organs were observed by using the body mass and organ index of mice as indexes and pathological sections of different organs.Results:The hypnotic experiment model of chloral hydrate showed that both natural and synthetic cinnabar(16.67 mg/kg,83.33 mg/kg)could prolong the sleep duration of mice induced by chloral hydrate,indicating that both natural and synthetic cinnabar had hypnotic effects.At a dose of 600 times the maximum dose(48 g/kg)of clinical medication administered by gavage,both types of cinnabar had no acute toxic effects on the mice,and there was no difference in mouse body weight,organ index,or toxicity to any organ compared to the blank group.Conclusion:The above research indicates that both natural cinnabar and synthetic cinnabar have hypnotic effects,and there is no significant difference in efficacy.Both types of cinnabar have no significant acute toxic effects.