枸杞叶对大鼠生长性能、血清生化与抗氧化指标及血浆代谢组的影响

Effects of Lycium barbarum Leaves on Growth Performance,Serum Biochemical and Antioxidant Indexes and Plasma Metabolome of Rats

本试验以大鼠为模式动物,旨在探究枸杞叶对动物生长性能、血清生化与抗氧化指标及血浆代谢组的影响。选取体重相近的健康雄性SD大鼠25只,随机分为5组(每组5个重复,每个重复1只):NC组(对照组)大鼠饲喂基础饲粮,PC组(枸杞果实阳性对照组)大鼠在饲喂基础饲粮的基础上灌服4 g/kg BW的枸杞果实匀浆,LD组、MD组和HD组(枸杞叶试验组)大鼠在饲喂基础饲粮的基础上分别灌服2、4和8 g/kg BW枸杞叶匀浆。预试期7 d,正试期28 d。结果显示:1)LD组大鼠的末重(338.63 g)和平均日增重(6.25 g/d)最高,显著高于NC组、PC组和MD组(P<0.05)。2)随枸杞叶匀浆灌胃剂量的上升,血清白球比降低,且HD组显著低于其他组(P<0.05)。3)LD组血清总抗氧化能力显著高于其他组(P<0.05),且随枸杞叶匀浆灌胃剂量的升高,血清总抗氧化能力显著下降(P<0.05)。4)通过血浆代谢组学分析共筛选出31种差异代谢物,富集的代谢通路主要有花生四烯酸代谢、嘌呤代谢、氨基酸代谢及烟酸和烟酰胺代谢等。综上所述,枸杞叶可提高模式动物大鼠的生长性能,并可能通过花生四烯酸代谢、氨基酸代谢等途径及中间代谢产物调控机体的抗氧化能力与免疫功能。本试验条件下,枸杞叶匀浆灌胃剂量为2 g/kg BW时效果较好。

The aim of this experiment was to investigate the effects of Lycium barbarum(L.barbarum)leaves on growth performance,serum biochemical and antioxidant indexes and plasma metabolome of rats.Twenty-five healthy male SD rats were selected and randomly divided into five groups with five rats according to the principle of similar body weight,and each rat as one replicate.The rats in NC group(control group)were fed a basal diet,and those in PC group(L.barbarum fruit positive control group)were fed the basal diet with 4 g/kg BW of L.barbarum fruit homogenate by gavage,while rats in LD,MD and HD groups(L.barbarum leaves test groups)were fed the basal diet with 2,4 and 8 g/kg BW of L.barbarum leaves homogenate,respectively.The pre-testing period was 7 d,and the formal test period was 28 d.The results showed as follows:1)LD group had the highest final weight(338.63 g)and average weight gain(6.25 g/d),which significantly higher than those in the NC,PC and MD groups(P<0.05).2)With the increase of L.barbarum leaves homogenate gavage dose,serum albumin/globulin(A/G)in HD group was the lowest,and significantly lower than that in other groups(P<0.05).3)Compared with other groups,serum total antioxidant capacity in LD group was significantly increased(P<0.05),and it decreased significantly with the increase of L.barbarum leaves homogenate gavage dose(P<0.05).4)A total of 31 differential metabolites were screened by plasma metabolomics analysis,main enrichment the metabolic pathways of arachidonic acid metabolism,purine metabolism,amino acid metabolism,and nicotinate and nicotinamide metabolism,etc.In summary,L.barbarum leaves can improve the growth performance of model animal rats,it may regulate antioxidant capacity and immune function through metabolic pathways such as arachidonic acid and amino acids and intermediate metabolites.Under the conditions of this experiment,the gavage dose of L.barbarum leaves homogenate with 2 g/kg BW has the better effect.