COPD患者lncRNA MIR155HG表达与肺功能的相关性及其对AECODP的辅助诊断价值

Correlation between lncRNA MIR155HG and pulmonary function in patients with COPD and its auxiliary diagnosis value for AECODP

目的 探讨慢性阻塞性肺疾病(COPD)患者长链非编码RNA(lnc RNA) MIR155HG与肺功能的相关性,及其对慢性阻塞性肺疾病急性加重(AECOPD)的辅助诊断价值。方法 选取2018年10月—2021年3月南阳市中心医院COPD患者117例,其中稳定期患者60例(稳定期组)、AECOPD患者57例(AECOPD组),以60名健康体检者作为正常对照组。收集所有研究对象一般资料,并检测肺功能[第1秒用力呼气容积(FEV1)、用力肺活量(FVC),计算FEV1/FVC%和外周血单个核细胞(PBMC)中lnc RNAMIR155HG的表达。采用Logistic回归分析评估AECOPD的危险因素。采用受试者工作特征(ROC)曲线评价各项指标诊断AECOPD的效能。结果 AECOPD组、稳定期组、正常对照组白细胞(WBC)计数、中性粒细胞百分比(NEUT%)、降钙素原(PCT)、C反应蛋白(CRP)、纤维蛋白原(Fib)、PBMC中lnc RNA MIR155HG相对表达量依次降低(P<0.05),FEV1和FEV1/FVC%依次升高(P<0.05)。稳定期组和AECOPD组PBMC中lnc RNA MIR155HG相对表达量与WBC计数、NEUT%、PCT、CRP和Fib水平均呈正相关(P<0.001),与FEV1和FEV1/FVC%均呈负相关(P<0.001)。Logistic回归分析结果显示,lnc RNAMIR155HG表达升高、FEV1降低是发生AECOPD的危险因素[比值比(OR)值分别为2.381、0.682,95%可信区间(CI)分别为1.526~3.715、0.531~0.876]。ROC曲线分析结果显示,FEV1、lnc RNA MIR155HG单项和联合检测诊断AECOPD的曲线下面积(AUC)分别为0.826、0.854、0.939。结论 COPD患者PBMC中lnc RNA MIR155HG水平升高,且与炎症指标和肺功能相关,或可作为AECOPD的早期诊断标志物。

Objective To investigate the correlation between long non-coding RNA(lncRNA)MIR155HG and pulmonary function in patients with chronic obstructive pulmonary disease(COPD)and its auxiliary diagnosis value for acute exacerbation of chronic obstructive pulmonary disease(AECOPD).Methods A total of 117 COPD patients treated in Nanyang Central Hospital from October 2018 to March 2021 were enrolled,which included 60 patients in stable phase as stable phase group and 57 patients with AECOPD as AECOPD group,and 60 healthy subjects were enrolled as control group.The general data were collected.Pulmonary function[forced expiratory volume in one second(FEV1),forced vital capacity(FVC)]was evaluated,and FEV1/FVC%was calculated.The level of lncRNA MIR155HG in peripheral blood mononuclear cell(PBMC)was determined.Logistic regression analysis was used to analyze the factors of AECOPD.Receiver operating characteristic(ROC)curve was used to analyze the efficiency of various indicators in the diagnosis of AECOPD.Results White blood cell(WBC)count,neutrophil percentage(NEUT%),procalcitonin(PCT),C-reactive protein(CRP),fibrinogen(Fib)and lncRNA MIR155HG level in PBMC were decreased in turn in AECOPD group,stable phase group and control group(P<0.05).FEV1 and FEV1/FVC%were increased in turn(P<0.05).The level of lncRNA MIR155HG in PBMC in AECOPD group and stable phase group was positively correlated with WBC count,NEUT%,PCT,CRP and Fib(P<0.001),and they were negatively correlated with FEV1 and FEV1/FVC%(P<0.001).Increased lncRNA MIR155HG and decreased FEV1 were risk factors for AECOPD[odds ratios(OR)were 2.381,0.682,95%confidence intervals(CI)were 1.526-3.715,0.531-0.876].The areas under curves(AUC)of FEV1 and lncRNA MIR155HG single and combined determinations to predict AECOPD were 0.826,0.854 and 0.939,respectively.Conclusions The increased level of lncRNA MIR155HG in PBMC of COPD patients is related to clinical indicators and pulmonary function,which is expected to become an early predictive marker of AECOPD.