射干制剂对失眠大鼠自主活动及TLR7/MyD88信号通路的影响

Effects of blackberry lily rhizome preparation on spontaneous activity and Toll-like receptor 7 gene/myeloid differentiation factor 88 signaling pathway in insomnia rats

目的 探研射干制剂对PCPA致失眠大鼠自主活动、脑电波以及脑组织TLR7、MyD88基因表达的影响。方法 随机将70只健康SD大鼠分为空白对照组、模型对照组、射干制剂低剂量组(26 mg·kg^(-1))、射干制剂中剂量组(52 mg·kg^(-1))、射干制剂高剂量组(104 mg·kg^(-1))、朱砂安神丸组(1.62 g·kg^(-1))、地西泮片组(0.9 mg·kg^(-1))。荧光定量PCR检测各组TLR7、My D88基因的相对表达量;采用Etho Vision大小鼠行为学系统记录大鼠自主活动情况;通过无线遥测系统记录各组睡眠时脑电图。结果 与空白组对照比较,模型对照组脑组织TLR7、MyD88相对表达量显著升高(P<0.01);同模型对照组相比,射干制剂低剂量组、射干制剂中剂量组TLR7、MyD88的mRNA表达量均降低(P<0.05),射干制剂高剂量组TLR7、MyD88 mRNA表达量显著降低(P<0.01);与空白对照组相比,PCPA造模后大鼠运动量明显增加,不同剂量射干制剂组、朱砂安神丸组及地西泮片组运动有不同程度的减少,射干制剂高剂量组运动减少较为明显;给药120 min后,与空白对照组相比,模型对照组脑电波中的δ波百分比降低(P<0.05);与模型对照组相比,射干低中剂量组、朱砂安神丸组、地西泮片组δ波百分比均升高(P<0.05)。结论 射干制剂可有效抑制大鼠兴奋性,增加脑电图δ波百分比,改善睡眠,这些作用可能是通过调节脑组织TLR7和MyD88基因的表达来实现。

Objective To explore the effects of blackberry lily rhizome preparation on spontaneous activity,electroencephalogram(EEG),and Toll-like receptor 7 gene(TLR7)and myeloid differentiation factor 88(MyD88)gene expression in brain tissue of PCPA-induced insomnia rats.Methods 70 healthy SD rats were randomly divided into the blank control group,the model control group,blackberry lily rhizome preparation low dose group(26 mg·kg),middle dose group(52 mg·kg"),high dose group(104 mg·kg^(-1)),cinnabar anshen pill group(1.62 g·kg^(-1))and Diazepam tablet group(0.9 mg·kg^(-1)).The relative expression levels of TLR7 and MyD88 genes in each group were detected by Quantitative Real-Time PCR(qRT-PCR).Spontaneous activity of rats was recorded by Etho Vision rat behavioral system.EEG of rats during sleep in each group was recorded by radio telemetry system.Results Compared with the blank control group,the relative expression levels of TLR7 and MyD88 in the brain tissue of the model control group were significantly increased(P<0.01).Compared with the model control group,the mRNA expression levels of TLR7 and MyD88 in blackberry lily rhizome preparation low dose group and middle dose group decreased(P<0.05),and the mRNA expression levels of TLR7 and MyD88 in high dose group significantly decreased(P<0.01).Compared with the blank control group,the exercise amount of rats after PCPA modeling increased significantly;The exercise amount of diferent doses of blackberry lily rhizome preparation group,cinnabar anshen pill group and Diazepam tablet group was decreased in different degrees,and the exercise amount of blackberry lily rhizome preparation high dose group was decreased significantly.After 120 minutes of administration,compared with the blank control group,the percentage of delta wave in EEG of the model control group decreased(P<0.05);Compared with the model control group,the percentage of delta wave in EEG was increased in blackberry lily rhizome preparation low dose group and middle dose group,cinnabar anshen pill group,and Diazepam tablet group(P<0.05).Conclusion Blackberry lily rhizome preparation can effectively inhibit excitability,increase the percentage of delta wave in EEG,and improve sleep in rats.And this effect may be achieved by regulating the expressions of TLR7 and MyD88 genes in brain tissue.