摘要
目的本研究旨在探讨芹菜素与白藜芦醇联合用药对非酒精性脂肪肝病(nonalcoholic fatty liver disease,NAFLD)的疗效。方法将50只雄性C57BL/6小鼠随机分为正常组10只和造模组40只,CCl4和高脂饲料诱导造模组建立NAFLD模型。造模成功后,将造模组进一步分为模型组、芹菜素组、白藜芦醇组、芹菜素与白藜芦醇联合组(联合给药组),每组10只,每天给药1次,连续4周。给药结束称量各组小鼠体质量,眼球取血后断颈处死,剖取肝脏称重,计算肝指数。采用全自动生化分析仪检测小鼠血清生化指标丙氨酸转氨酶(alanine transaminase,ALT)、天冬氨酸转氨酶(aspartate transaminase,AST)、三酰甘油(triacylglycerol,TG)、总胆固醇(total cholesterol,TC)水平,酶联免疫吸附测定法(enzyme-linked immunosorbent assay,ELISA)检测小鼠10%肝组织匀浆液中超氧化物歧化酶(superoxide dismutase,SOD)、丙二醛(malondialdehyde,MDA)、过氧化氢酶(catalase,CAT)和谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)含量。HE染色观察小鼠肝脏组织病理形态学状况。结果与正常组相比,模型组肝指数和血清生化ALT、AST、TG、TC水平显著升高(P<0.01),肝匀浆MDA显著升高(P<0.01),SOD、CAT、GSH-Px水平显著下降(P<0.01),肝组织存在大量脂肪空泡及肝索紊乱现象。与芹菜素组、白藜芦醇组相比,联合给药组肝指数以及血清生化ALT、AST、TG和TC水平降低(P<0.05),肝匀浆MDA水平下降(P<0.05),SOD、CAT、GSH-Px水平升高(P<0.05),肝组织脂肪空泡减少,肝索紊乱现象较改善。结论芹菜素与白藜芦醇联合给药可对NAFLD模型小鼠具有保护作用,其作用机制可能与降低肝内酶和血脂水平、增加抗氧化作用有关,作用好于芹菜素组和白藜芦醇组。
Objective To investigate the efficacy of combined use of apigenin and resveratrol in the treatment of nonalcoholic fatty liver disease(NAFLD).Methods Fifty male C57BL/6 mice were randomly divided into a normal group(n=10)and a model group(n=40).The NAFLD model was established in the model group using carbon tetrachloride(CCl4)and a highfat diet.After successful modeling,the model group was further divided into a model group,an apigenin group,a resveratrol group,and an apigenin and resveratrol combined group(combined administration group),with 10 mice in each group.The mice were administered once daily for four consecutive weeks.At the end of the administration,the mice in each group were weighed,the eyeballs were taken for blood samples,and the necks were dissected and sacrificed.The livers were dissected and weighed,and the liver index was calculated.The automatic biochemical analyzer was used to detect the serum biochemical indicators of alanine aminotransferase(ALT),aspartate aminotransferase(AST),triglycerides(TG),and total cholesterol(TC)levels in mice.The ELISA method was used to detect the contents of superoxide dismutase(SOD),malondialdehyde(MDA),catalase(CAT)and glutathione peroxidase(GSH-Px)in mouse 10%liver tissue homogenate.HE staining was used to observe the pathological morphology of liver tissue in mice.Results Compared with the normal group,the liver index and serum biochemical ALT,AST,TG,TC levels in the model group were significantly increased(P<0.01),and the liver homogenate MDA was significantly increased(P<0.01),while the levels of SOD,CAT,GSH-Px were decreased significantly(P<0.01).There were a large number of fatty vacuoles and hepatic cord disorders in the liver tissue.Compared with the apigenin group and the resveratrol group,the liver index,and serum biochemical ALT,AST,TG and TC levels in the combined administration group decreased(P<0.05),and the liver homogenate MDA level decreased(P<0.05),and the levels of SOD,CAT and GSH-Px were increased(P<0.05).The number of fatty vacuoles in liver tissues were reduced,hepatic cord disorders were improved.Conclusions The combined administration of apigenin and resveratrol has a protective effect on NAFLD model mice,possibly through the reduction of hepatic enzymes and blood lipid levels,as well as enhanced antioxidant activity.The combination treatment shows better efficacy compared to the apigenin and resveratrol groups.
作者
付海洋
韩伟东
张海云
FU Haiyang;HAN Weidong;ZHANG Haiyun(Nantong Sixth People's Hospital,Nantong,Jiangsu 320611,China)
出处
《中国热带医学》
CAS
北大核心
2024年第1期70-74,共5页
China Tropical Medicine
基金
国家自然科学基金项目(No.21302223)
南通市科技局指导项目(No.JCZ20006)。
关键词
非酒精性脂肪肝病
芹菜素
白藜芦醇
氧化应激
Non-alcoholic fatty liver disease
apigenin
resveratrol
oxidative stress