UPLC-MS/MS法测定糕点中胭脂红酸的含量

Determination of Carminic Acid in Pastries by Ultra High Performance Liquid Chromatography-Triple Quadrupole Tandem Mass Spectrometry

建立了一种简便、高效、准确的测定糕点中胭脂红酸的超高效液相色谱串联三重四极杆质谱法。样品以2 mol·L^(-1)盐酸水溶液提取,HLB柱净化,氮吹至近干后,用2%甲酸水溶液-甲醇(1+1,v/v)复溶后上机测定。结果表明,胭脂红酸在1~200 ng·mL^(-1)浓度范围内线性良好,相关系数> 0.999,方法检出限为1.10 ng·g^(-1),定量限为3.85 ng·g^(-1)。在100 ng·g^(-1)和250 ng·g^(-1)加标水平下,回收率在91.1%~98.0%,相对标准偏差均<10%。本方法快捷、灵敏、准确,可用于糕点中胭脂红酸的检测。

A simple,efficient and accurate ultra high performance liquid chromatography-triple quadrupole tandem mass spectrometry for the determination of carminic acid in pastries was developed.The sample was extracted with 2 mol·L~(-1) hydrochloric acid aqueous solution,purified by an HLB column,nitrogen-blown to near dryness,and then re-dissolved with 2% formic acid aqueous solution-methanol(1+1,v/v) for determination.The results showed that the linearity of cochineal acid was good in the concentration range of 1 ~ 200 ng·mL~(-1) with the correlation coefficient > 0.999.The limit of detection of the method was 1.10 ng·g~(-1),and the limit of quantification was 3.85 ng·g~(-1).The recoveries were in the range of 91.1% ~ 98.0% at the spiked levels of 100 ng·g~(-1) and 250 ng·g~(-1),and the relative standard deviations were all < 10%.The method is rapid,sensitive,accurate and can be used for the determination of carminic acid in pastries.