益口葛葛水提物对抑郁-焦虑小鼠模型的作用研究

Study on the Effect of Water Extract of Yikou Gege on Depression-Anxiety Mice Model

目的:研究由葛根制备的槟榔风味的益口葛葛咀嚼块水提物对小鼠抗抑郁以及口腔损伤的影响。方法:将48只昆明小鼠随机分为空白对照组、槟榔组、葛根组、益口葛葛组4组。空白组正常饲养,干预组用相应的水提物(10 mg·mL^(-1))涂抹小鼠口腔内壁,左右各15次,涂抹完后禁饮1 h。4周后对小鼠进行4周的慢性不可预知温和刺激抑郁模型(Chronic Unpredictable Mild Stimulation,CUMS)造模,期间涂抹不中断,然后用小鼠旷场实验(Open Field Test,OFT)、悬尾实验(Tail Suspension Test,TST)、小鼠强迫游泳实验(Forced Swimming Test,FST)以及糖水偏好实验(Sucrose Preference Test,SPT)评价各组的抗抑郁活性。处死小鼠,比较各组脏器指数,对口腔黏膜、肝进行病理学染色,提取口腔组织RNA检测口腔纤维化关键基因表达情况。结果:相比于空白组,仅葛根组体重下降,葛根的加工制品可逆转其体重下降,槟榔组可显著降低悬尾、强迫游泳实验小鼠不动时间(P <0.001,P <0.001),各组在自发活动、糖水偏好上无统计学差异。病理染色上,仅槟榔组的口腔黏膜存在明显的免疫细胞浸润和胶原纤维增生,肝组织无明显差异。结论:槟榔有较好的改善抑郁趋势,但存在明显的口腔损伤,而益口葛葛缓解抑郁的趋势虽然比槟榔稍弱,但不会损伤口腔健康。

Objective:To study the effects of water extract of bequel-flavored Yikou Gege chewblock prepared from Pueraria lobata on anti-depression and oral injury in mice.Method:48 Kunming mice were randomly divided into four groups:blank control group,betel nut group,Gegen group and Yikou Gege group.Normal feeding blank group,intervention group with the corresponding aqueous extract(10 mg·mL~(-1)) daub mouse oral cavity wall,about 15 times each,daub after the forbidden drink 1 h.Four weeks later,the mice were subjected to 4 weeks of depression induced by chronic unpredictable mild stimulation(CUMS),with continuous application during this period.The antidepressant activity of the mice was evaluated by open field test(OFT),tail suspension test(TST),forced swimming test(FST) and sucrose preference test(SPT).The mice were sacrificed,and the organ index of each group was compared.The pathological staining of oral mucosa and liver was performed,and RNA was extracted from oral tissue to detect the expression of key genes in oral fibrosis.Result:Compared with the blank group,only the Pueraria lobata group lost weight,and the processed products of Pueraria lobata could reverse the weight loss.The betel nut group could significantly reduce the immobility time of the tail suspension and forced swimming test mice(P<0.001,P < 0.001),and there was no significant difference in spontaneous activity and sucrose preference among the groups.In the pathological staining,only the betel nut group had obvious immune cell infiltration and collagen fiber hyperplasia in the oral mucosa,but there was no significant difference in the liver tissue.Conclusion:Betel quid has a better tendency to improve depression,but there is obvious oral damage.Although the trend of alleviating depression is slightly weaker than that of betel quid,it does not damage oral health.