黔产八角枫HPLC指纹图谱研究

Study on HPLC fingerprint of Alangium chinense from Guizhou

目的:建立黔产八角枫药材指纹图谱,结合相似度评价、聚类、主成分分析评价黔产八角枫药材品质,为其质量研究提供技术方法借鉴和基础数据。方法:色谱柱采用HC-C 18(250 mm×4.6 mm,5μm),以甲醇-缓冲液(缓冲液:0.2 g庚烷磺酸钠,2.0 g磷酸二氢钾,0.3 mL磷酸,定容至1000 mL)为流动相进行梯度洗脱,运用中药色谱指纹图谱相似度评价系统(2012A版)进行相似度评价分析,采用SPSS26.0软件进行主成分分析和聚类分析。结果:建立了黔产八角枫药材指纹图谱,综合10个批次样品的色谱图谱,标定了8个共有峰,指认了八角枫碱色谱峰。10批次药材的相似度在0.801~0.994之间,主成分分析结果表明,贵州遵义和毕节质量较优;聚类分析结果表明,类间距为15时,10份样品被分为3类,即产自凯里的2批八角枫药材聚为一类,产自毕节和遵义的2批聚为一类,其余6批聚为一类。结论:该方法准确,稳定,可靠,可为八角枫质量评价提供参考。

In this study we established the fingerprint of medicinal Alangium chinense from Guizhou,and evaluated the quality of A.chinense based on similarity evaluation,clustering and principal component analysis,so as to provide technical reference and basic data for the quality research of A.chinense.High performance liquid chromatography was adopted,the chromatographic column was HC-C 18(250 mm×4.6 mm,5μm),the mobile phase for gradient elution was methanol-buffer(buffer:0.2 g sodium heptane sulfonate,2.0 g potassium dihydrogen phosphate,0.3 mL phosphoric acid,diluted to 1000 mL),similarity evaluation was performed by using the traditional Chinese medicine chromatographic fingerprint similarity evaluation system(ver.2012A),and principal component analysis and clustering analysis was performed by using SPSS26.0 software.The HPLC fingerprint of A.chinense was established,the chromatograms of 10 batches of samples were synthesized,8 common peaks were calibrated,and the peak of alangine was identified.The similarity of the 10 batches of samples was between 0.801 and 0.994.The principal component analysis showed that the quality of A.chinense from Zunyi and Bijie was better.The clustering analysis showed that when the class distance was 15,the 10 samples were divided into 3 categories:the 2 batches from Kaili were clustered into one category,the 2 batches from Bijie and Zunyi were clustered into one category,and the other 6 batches were clustered into one category.This method is accurate,stable and reliable,and can provide reference for the quality evaluation of A.chinense.