泽泻醇B对HepG2细胞体外糖吸收的影响初探

Preliminary Study on the Effect of Alisitol B on Glucose Uptake in HepG2 Cells in vitro

目的:探索泽泻醇B对HepG2细胞糖吸收的影响。方法:以不同浓度的泽泻醇B处理HepG2细胞,经CCK-8法(Cell Counting Kit-8细胞计数试剂,CCK-8),评估泽泻醇B对细胞增殖的影响。将不同培养基培养的HepG2细胞分别采用药物处理后,加入2-NBDG(2-[N-7-硝基苯并-2-氧杂-1,3-二唑-4-氨基]-2-脱氧葡萄糖,2-NBDG)为葡萄糖荧光示踪剂,用流式细胞仪测定细胞荧光强度,以此评估泽泻醇B对HepG2细胞糖吸收的影响。结果:CCK-8实验结果表明:在1~10μmol/L范围内,泽泻醇B对HepG2细胞增殖的情况影响较小。糖吸收测定实验结果表明:在模拟的条件下,泽泻醇B表现出对HepG2细胞糖吸收有不同程度的促进作用。结论:初步确定泽泻醇B可促进HepG2细胞的糖吸收,提示了泽泻醇B可能是泽泻降血糖的有效成分之一。

Objective To investigate the effect of Alisol B on glucose absorption in HepG2 cells.Methods HepG2 cells were treated with different concentrations of AlisolB.Cell absorbance values were measured by CCK-8 method(cell Counting Kit-8 cell counting reagent,CCK-8)to evaluate the effects of Alisol B on cell proliferation.HepG2 cells cultured indifferent mediawere treated with drugs,and 2-NBDG(2-[N-7-nitrobenzo-2-oxa-1,3-diazo-4-amino]-2-deoxyglucose,2-NBDG)was added as glucose fluorescent tracer,and the cell fluorescence intensity was measured by flow cytometry at a wavelength to evaluate the effect of Alisol B on glucose uptake in HepG2 cells.Results The results of CCK-8 experiments showed that in the range of 1~10μmol/L,Alisol Bhad little effect on the value-added of HepG2 cells.The results of glucose absorption assay showed that Alisol B could promote the glucose absorption of HepG2 cells in different degrees under four simulated experimental environments.Conclusion It was preliminarily confirmed that Alisol B had a positive effect on the glucose absorption of HepG2 cells,and it was verified that Alisol B was one of the active ingredients for HepG2 cells in hypoglycemic.