基于生物信息学方法分析脑胶质瘤组织中糖蛋白M6A的表达

Analysis of the Expression of Glycoprotein M6A in Glioma based on Bioinformatics Methods

目的探讨糖蛋白M6A(glycoprotein M6A,GPM6A)在脑胶质瘤标本中的表达及其临床意义。方法运用生物信息学方法分析中国脑胶质瘤基因组图谱(Chinese Glioma Genome Atlas,CGGA)数据库325例患者GPM6A表达情况及其与临床病理参数关系。运用STRING在线数据库筛选分析GO功能注释以及与GPM6A相互作用的可能蛋白-蛋白作用功能网络。利用TIMER2.0数据库评估GPM6A与胶质瘤细胞纯度及浸润性CD8+T细胞水平之间的关系。结果CGGA数据库在线分析显示,在各病理组织学分级(WHO:G_(2)、G_(3)、G_(4)级)与各组织学病理分型中均有GPM6A表达,各分级或各分型脑胶质瘤的GPM6A表达水平比较,差异均有统计学意义(分级:F=50.25,P<0.001;分型:F=14.26,P<0.001);GPM6A表达水平与分子IDH突变状态、1p19q联合缺失以及患者年龄密切相关,差异有统计学意义(P均<0.01),但与患者性别无关(t=1.45,P>0.05)。GPM6A表达水平在原发性胶质瘤中稍高于继发性胶质瘤,差异无统计学意义(t=1.82,P>0.05)。患者生存期数据分析显示,在低级别胶质瘤中GPM6A低表达的患者生存期较GPM6A高表达患者明显缩短(χ^(2)=69.79,P<0.001),但GBM患者中,无论GPM6A表达水平高低,生存期差异无统计学意义(χ^(2)=0.63,P>0.05)。GO功能分析显示,GPM6A与神经系统中跨膜蛋白运输、突触形成与发育、细胞之间信息交流等功能密切相关。TIMER2.0数据库分析显示GPM6A与胶质瘤肿瘤细胞纯度和浸润的CD8+T细胞水平正相关。结论GPM6A低表达胶质瘤患者预后显著差于高表达患者,其表达水平可作为评估胶质瘤预后的预测因素。

Objective To investigate the expression of glycoprotein M6A(GPM6A)in glioma specimens and its prognostic significance.Methods Bioinformatics methods were used to analyze the expression of GPM6A in 325 cases obtained from the Chinese Glioma Genome Atlas(CGGA)database.The STRING online database was used to screen and analyze the possible protein PPI interaction network and GO functional annotation that interact with GPM6A to explore its potential mechanism.The relationships between GPM6A and glioma cell purity and the level of CD8+T cell were assessed using the TIMER2.0database.Results The online analysis of the CGGA database showed that GPM6A was expressed in histopathological grades(WHO:G_(2),G_(3),G_(4))and histopathological types,and there were significant differences in GPM6A expression levels between all grades and all types of gliomas(Grades:F=50.25,P<0.001;Types:F=14.26,P<0.001);the expression level of GPM6A was closely related to molecular IDH mutation status,combined deletion of 1p19q,and patient age(all P<0.01),regardless of patient gender(t=1.45,P>0.05).The expression level of GPM6A in primary glioma was higher than that in secondary glioma,and the difference was not statistically significant(t=1.82,P>0.05).The survival time of patients with low GPM6A expression was significantly shortened(χ^(2)=69.79,P<0.001),but in glioblastoma(GBM)patients,regardless of the level of GPM6A expression,there was no difference in survival time(χ^(2)=0.63,P>0.05).GO functional analysis showed that GPM6A was closely related to the functions of transmembrane protein transport,synapse formation and development,and information exchange between cells in the nervous system.TIMER2.0database analysis showed that GPM6A was positively correlated with glioma tumor cell purity and the level of CD8+T cell.Conclusion The prognosis of glioma patients with low GPM6A expression is significantly worse than that of patients with high expression,and its expression level can be used as a predictor for evaluating the prognosis of glioma.