北沙参种子内源抑制物质活性研究

研究北沙参种子和果皮各分离相对白菜种子萌发和幼苗的影响,探讨北沙参种子内源抑制物的活性。对北沙参种子不同部位的浸提液通过系统溶剂法进行分离,分离出石油醚相、二氯甲烷相、乙酸乙酯相、正丁醇相、甲醇相和水相,并以各分离相对白菜种子进行萌发实验,测定北沙参种子内源抑制物生物活性。结果显示,①北沙参种子、果皮各分离相对白菜种子萌发均有一定的抑制作用,其中抑制作用最强的是甲醇相,其次是乙酸乙酯相;与对照组相比,各处理组的发芽指数均有一定程度的下降;甲醇相的平均发芽天数显著长于其他处理。②北沙参种子、果皮各分离相在抑制白菜幼苗根长和芽长方面表现出明显的差异,其中,甲醇相的抑制作用最显著,其次为乙酸乙酯相。与萌发指标结果一致。北沙参种子不同部位分离相对白菜种子萌发及幼苗存在不同程度的抑制作用,表明北沙参种子、果皮均含有高活性抑制物质,主要存在于甲醇相和乙酸乙酯相,因此,北沙参种子中的内源抑制物是导致其休眠的主要原因。

The effects of seed and pericarp isolation of Radix Glehniae(aks.CoasiaI GIehnia Root)on seed germination and seedlings of Chinese cabbage were studied to explore the activity of endogenous inhibitors in seeds of Radix Glehniae.The extracts from different parts of the seeds of Radix Glehniae were separated by systematic solvent method,and the petroleum ether phase,dichloromethane phase,ethyl acetate phase,n-butanol phase,methanol phase and water phase were separated,and the germination experiments were carried out relative to Chinese cabbage seeds.The biological activity of endogenous inhibitors in Radix Glehniae seeds was determined.The results showed that:①The separation of seeds and pericarps of Radix Glehniae had a certain inhibitory effect on the germination of Chinese cabbage seeds,in which methanol phase was the strongest,followed by ethyl acetate phase.Compared with the control group,the germination index of each treatment group decreased to a certain extent,and the average germination days of methanol phase was significantly longer than that of other treatments.②The separated phases of seeds and pericarps of Radix Glehniae showed obvious differences in inhibiting root length and bud length of Chinese cabbage seedlings,among which methanol phase was the most significant,followed by ethyl acetate phase.The results were consistent with the germination index.The isolation of different parts of the seeds of Radix Glehniae had different inhibitory effects on the seed germination and seedlings of Chinese cabbage,which indicated that the seeds and pericarps of Radix Pseudostellariae contained highly active inhibitors,which mainly existed in methanol phase and ethyl acetate phase.The endogenous inhibitor in the seeds of Radix Glehniae is the main reason for its dormancy.