金针菇MNP分子标记菌株鉴别技术建立

Establishment of MNP molecular identification technology for Flammulina filiformis strains

金针菇Flammulina filiformis是受《植物新品种保护名录》保护的食用菌之一,品种的知识产权保护受到高度重视,迫切需要准确、可靠、稳定和可操作性强的品种鉴别技术为品种知识产权保护提供技术支撑。本研究利用42株金针菇菌株基因组重测序数据构建了包含428个多核苷酸多态性(MNP)分子标记的数据库,并对试验菌株之间的遗传相似度进行分析。结果显示来自国内金针菇企业所用的12个菌株遗传相似度均为100%,暗示国内金针菇生产品种高度同质化;而12个野生菌株间的遗传相似度介于0.23%–29.44%之间(除F17与F18的相似度为100%外),体现了野生金针菇具有丰富的遗传多样性。此外,MNP分子标记的鉴别结果还与拮抗实验和ISSR分子标记的结果进行了比较,体现出MNP分子标记菌株鉴别技术的灵敏度高、稳定性好、工作量小的优势,因此可为我国金针菇品种知识产权保护提供技术支撑。

Flammulina filiformis is one of the edible fungi protected by the law of Protection List of New Varieties of Plants.The intellectual property protection of varieties is highly valued,and there is an urgent need for accurate,reliable,stable,and operable variety identification technologies to provide technical support for the protection of intellectual property rights of varieties.The study used the genome resequencing data of 42 F.filiformis strains to construct a database containing 428 polynucleotide polymorphism(MNP)molecular markers,and analyzed the genetic similarity between the test strains.The results showed that the genetic similarity of the 12 strains used by domestic cultivators was 100%,implying that domestic F.filiformis production varieties are highly homogeneous.The genetic similarity among the 12 wild strains ranged from 0.23%to 29.44%(except for F17 and F18,whose similarity was 100%),reflecting the rich genetic diversity of wild F.filiformis.The identification results of MNP molecular markers were also compared with the results of antagonistic experiments and ISSR molecular markers,reflecting the advantages of high sensitivity,good stability,and small workload of MNP molecular marker strain identification technology.Therefore,this identification technology can provide technical support for the intellectual property protection of F.filiformis varieties in China.