富含玉米黄质色素橄榄油对高血脂小鼠的影响

Effect of olive oil rich in zeaxanthin on hyperlipidemia mice

旨在为开发富含玉米黄质色素橄榄油(ZT)保健产品提供参考,用脂肪乳剂灌胃构建高血脂小鼠模型,同时设生理盐水对照(CK)组,将建模成功的小鼠随机分为高血脂模型组(Model),ZT高、中、低剂量处理组和菲勒贝特药物对照(PL)组,连续灌胃给药28 d,研究ZT(玉米黄质色素含量400 mg/100 g)对高血脂小鼠空腹血糖(FBG)、糖化血红蛋白(HbAlc)、总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白(HDL)、低密度脂蛋白(LDL)、总胆红素(TBIL)、总胆汁酸(TBA)、丙氨酸氨基转移酶(ALT)、天门冬酸氨基转移酶(AST)、γ-氨酰基转移酶(GGT)、碱性磷酸酶(ALP)等指标的影响,并对ZT小鼠开展急、慢性毒理研究。结果表明:ZT能有效降低高血脂小鼠的FBG、HbAlc、TC、TG、LDL、TBIL、TBA含量,升高HDL含量,对ALT、AST、GGT和ALP活性具有很好的抑制作用,能够显著改善血糖、血脂代谢紊乱,降低小鼠体质量,对正常小鼠无急、慢性毒理作用,可提高小鼠血清中超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和谷胱甘肽过氧化物酶(GSH-Px)的活性。综上,富含玉米黄质色素橄榄油属无毒性物质,能提高小鼠的抗氧化能力,降低其体质量,是具有较好辅助降血糖、降血脂作用的橄榄油保健食品。

In order to provide a reference for the development of health care products of olive oil rich in zeaxanthin(ZT),the mice model of hyperlipidemia was established by intragastric administration of lipid emulsion,and the nomal saline group(CK)was set up at the same time.The successfully modeled mice were randomly divided into hyperlipidemia model group(Model),high,middle and low dosages groups of ZT,and Philbert control group(PL).The drug was given by intragastic administration continuously for 28 d to investigate the effects of ZT(zeaxanthin content 400 mg/100 g)on fasting blood glucose(FBG),glycated hemoglobin(HbAlc),total cholesterol(TC),triglyceride(TG),high-density lipoprotein(HDL),low-density lipoprotein(LDL),total bilirubin(TBIL),total bile acid(TBA),alanine aminotransferase(ALT),aspartate aminotransferase(AST),gamma-ammonia acyltransferase(GGT)and alkaline phosphatase(ALP)in hyperlipemia mice,and acute and chronic toxicological studies on ZT mice were carried out.The results showed that ZT could effectively decrease the contents of FBG,HbAlc,TC,TG,LDL,TBIL,TBA,increase the content of HDL,and inhibit the activities of ALT,AST,GGT and ALP.It could significantly improve the metabolic disorder of blood glucose and lipid,and reduce the body weight of mice.It had no acute or chronic toxicity to normal mice.It could increase the activities of SOD,CAT and GSH-Px in serum of mice.Therefore,olive oil rich in zeaxanthin is a non-toxic substance,which can improve the antioxidant capacity and reduce the body weight of mice,and it is an olive oil health-care food with good auxiliary blood glucose reduction and blood lipid reduction effects.