肾活检组织Masson染色方法的改良

Improvement of Masson staining method for renal biopsy tissue

目的 通过对传统Masson染色方法的改良,解决肾活检光镜诊断中Masson染色存在的色彩浑浊,定位不清等问题。方法 收集2021-2022年的25例膜性肾病标本,分别采用改良染色法与传统方法,对比染色质量进行评分。结果 25例膜性肾病4种Masson染色结果比较,发现变色酸2R-亮绿+Bouin二次固定+胰酶消化法在色彩鲜明、嗜复红蛋白定位清晰及不易褪色、切片整体评分方面均优于传统方法,差异有统计学意义(P<0.05)。结论 改良后的Masson的染色方法在色彩对比度、持久度均优于传统方法。

Objective To improve the traditional Masson staining method and solve the problems of cloudy color and unclear localization in the diagnosis of renal biopsy under light microscopy.Methods Twentyfive samples of membranous nephropathy from 2021 to 2022 were collected,and the improved staining method and traditional method were used to compare the staining quality and score.Results Comparing the results of four Masson staining methods in 25 cases of membranous nephropathy,it was found that the Chromogenic acid 2R&Bright green & Bouin secondary fixation & Pancreatic enzyme digestion method showed significant differences in color,clear localization of eosinophil,and non-fading ability compared to traditional methods(P<0.05).Conclusion The improved Masson staining method outperforms traditional methods in terms of color contrast and persistence.