溃疡性结肠炎患者血清Chemerin与Th9/Treg细胞失衡的相关性分析

Correlation analysis of serum Chemerin level and Th9/Treg cell imbalance in pa-tients with ulcerative colitis

目的探究溃疡性结肠炎(ulcerative colitis,UC)患者血清趋化素(Chemerin)水平与Th9/Treg细胞失衡的相关性。方法选取2020年11月至2021年11月于我院确诊为UC的患者85例(UC组)和健康体检者80名(对照组)。流式细胞术检测Th9/Treg的比例;采用ELISA法检测血清中IL-9、CRP、IL-10、IL-17的含量。统计学分析Chemerin表达与Mayo评分的相关性;与Th9、Treg、Th9/Treg以及相关细胞因子之间的相关性。结果UC组Chemerin水平和Mayo评分均显著高于对照组,UC患者重度组Chemerin水平和Mayo评分均显著高于中度组和轻度组,中度组显著高于轻度组(P<0.05);Chemerin表达与Mayo评分之间呈正相关(P<0.05);流式细胞术结果显示,UC患者血清中Th9/CD4+的比例显著升高,Treg/CD4+比例显著降低,Th9/Treg比例显著升高(P<0.05);UC组Treg比例以及IL-10水平显著低于对照组,Th9比例、Th9/Treg以及IL-9、CRP、IL-17水平显著高于对照组(P<0.05);UC患者重度组的Treg比例、IL-10水平显著低于中度组和轻度组,中度组显著低于轻度组(P<0.05);UC患者重度组的Th9比例、Th9/Treg、IL-9、CRP、IL-17水平显著高于中度组和轻度组,中度组显著高于轻度组(P<0.05);UC组患者血清Chemerin表达与Th9比例、Th9/Treg、CRP、IL-17、IL-9水平呈正相关,与Treg比例、IL-10水平呈负相关(P<0.05)。结论UC患者血清Chemerin水平与Th9/Treg细胞失衡有密切关系。

Objective To explore the correlation between level of serum Chemerin and Th9/Treg imbalance in patients with ulcerative colitis(UC).[WTHZ]Methods From Nov.2020 to Nov.2021,85 patients who were diagnosed with UC in our hospital were taken as the UC group.Eighty healthy subjects were taken as the control group.Flow cytometry was applied to detect the ratio of Th9/Treg.ELISA was used to detect serum levels of IL-9,CRP,IL-10 and IL-17.The correlation between Chemerin expression and Mayo score,the correlation between Chemerin expression and Th9,Treg,Th9/Treg and related cytokines were analyzed.[WTHZ]Results The level of Chemerin and Mayo score in UC group were obviously higher than those in control group,the level of Chemerin and Mayo score in severe group were obviously higher than those in moderate group and mild group,and the moderate group were obviously higher than that in mild group(P<0.05).There was a positive correlation between Chemerin expression and Mayo score(P<0.05).The proportion of Treg and the level of IL-10 in the UC group were obviously lower than those in the control group,while the proportion of Th9,Th9/Treg and levels of IL-9,CRP,IL-17 in the UC group were obviously higher than those in the control group(P<0.05).The proportion of Treg and the level of IL-10 in the severe group of UC patients were obviously lower than those in the moderate and mild groups,and the moderate group were obviously lower than the mild group(P<0.05).Flow cytometry results showed that the ratio of Th9/CD4+in serum of UC patients was significantly increased,the ratio of Treg/CD4+was significantly decreased,and the ratio of Th9/Treg was significantly increased(P<0.05).The proportion of Th9,Th9/Treg,and the levels of IL-9,CRP,IL-17 in the severe group of UC patients were obviously higher than those in the moderate and mild groups,while the above values in the moderate group were obviously higher than those in the mild group(P<0.05).The expression of serum Chemerin in UC group was positively correlated with Th9 ratio,Th9/Treg,levels of CRP,IL-17,IL-9,and negatively correlated with Treg ratio and level of IL-10(P<0.05).[WTHZ]Conclusion Serum Chemerin level in UC patients is closely related to Th9/Treg cell imbalance.