8种食品水提液的抗氧化和抗炎活性比较

Comparative studies on the antioxidant and anti-inflammatory activities of water extracts from eight kinds of foods

目的:研究不同食品水提液的活性成分、抗氧化活性、抗炎活性及其相关性。方法:选择黑米、黑豆、黑芝麻、黑木耳、黑枣、黑玉米、燕麦和小米8种常见食品,测定其水提液中总黄酮与总多酚含量,并比较其活性成分差异;测定其1,1-二苯基-2-三硝基苯肼(DPPH)自由基清除能力、羟自由基清除能力及铁离子还原能力并比较其抗氧化活性;测定脂多糖(LPS)诱导的RAW264.7小鼠巨噬细胞中NO及IL-6抑制率,并比较其抗炎活性。结果:8种食品的抗氧化与抗炎活性均与其总黄酮和总多酚含量呈正相关,且两种活性间亦呈正相关性。其中,黑枣的总黄酮和总多酚含量最高,分别为1.716,3.043 mg/g;当样品质量浓度为1 mg/mL时,DPPH自由基清除能力高达89.48%,其抗氧化活性具有显著性优势;当样品质量浓度为5 mg/mL时,其对NO的抑制率为65.92%,对IL-6的抑制率达78.62%而表现出明显的抗炎活性。黑米具有较强的DPPH自由基清除能力与铁离子还原能力,同时对NO和IL-6表现出较强的抑制活性;而作为非黑色食品的小米,其活性虽不及黑枣与黑米,但较黑玉米、黑木耳等黑色食品,在羟自由基清除与抗炎活性上却表现明显的优势。结论:8种食品水提液均具有不同程度的抗氧化作用,也呈现出一定的抗炎活性,但有较大差异;其活性与总黄酮、总多酚含量呈正相关。其中,黑枣的总黄酮、总多酚含量最高,抗氧化、抗炎活性亦最强。

Objective:This study aimed to investigate the active ingredients,and their antioxidant and anti-inflammatory activities,as well as the correlation between each other of different food water extracts.Methods:Black rice,black soybean,black sesame,Auricularia auricula,diospyros lotus,black corn,oat and millet were selected for the comparison study of the content of total flavonoids and polyphenols in the aqueous extract,and then the 1-diphenyl-2-picrylhydrazyl(DPPH)scavenging activity,hydroxyl radical scavenging ability and iron ion reduction capacity were determined to evaluate their antioxidant activity.Furthermore,the NO and IL-6 inhibitory activities on Lipopolysaccharides(LPS)induced RAW264.7 cells were tested to investigate the anti-inflammatory activities.Results:The antioxidant and anti-inflammatory activities were positively correlated with the contents of total flavonoids and polyphenols,and the two activities were also positively correlated.Among them,diospyros lotus showed the highest content of total flavonoids and polyphenols with 1.716 mg/g and 3.043 mg/g respectively,and the radical clearance capacity of DPPH was 89.48%at 1 mg/mL which was significantly higher than the others.It also showed anti-inflammatory activity with 65.92%and 78.62%inhibitory rates against NO and IL-6 at 5 mg/mL on LPS induced RAW264.7 cells.Meanwhile,black rice had a strong DPPH scavenging ability and iron ion reducing ability,and it also showed strong inhibitory activities against NO and IL-6.Millet,a non-black food,showed obvious advantages in hydroxyl free radical scavenging activity and anti-inflammatory effect compared with black corn and A.auricula.Conclusion:Water extracts from eight kinds of food had varying degrees of antioxidant and anti-inflammatory activities which were positively correlated with the contents of total flavonoids and polyphenols.Among them,diospyros lotus had the highest content of total flavonoids and polyphenols with the strongest antioxidant and anti-inflammatory activities.Therefore,it is necessary to carry out more in-depth application research on diospyros lotus.