草菇子实体多肽对小鼠急性酒精肝的预防作用及肠道菌群的影响

Preventive Effect of Volvariella volvacea Fruit Body Polypeptides on Acute Alcoholic Liver Injury in Mice and Its Influence on Intestinal Microflora

为了探究草菇子实体多肽(Volvariella volvacea fruit body polypeptides,VVFP)对模型小鼠急性酒精肝预防作用及肠道菌群的影响,以先前获得的VVFP(分子质量1~3 kDa)为材料灌胃小鼠,将小鼠随机分为空白组、模型组、阳性对照组、多肽低剂量组、多肽中剂量组、多肽高剂量组,比较各组小鼠的血清和肝脏指标以及组织病理切片,利用16S rDNA基因高通量测序分析各样品中微生物菌群的生物多样性以及在门和属水平上的相对丰度。结果表明,与模型组相比,VVFP能够极显著降低血清中甘油三酯、总胆固醇、谷丙转氨酶、谷草转氨酶水平和肝脏中丙二醛的含量,并且降低肿瘤坏死因子-α、白细胞介素-6炎症因子水平,同时明显提高肝脏中乙醇脱氢酶、总超氧化物歧化酶和谷胱甘肽过氧化物酶的活性。16S rDNA基因高通量测序结果表明,VVFP可以使小鼠肠道菌群中α多样性的Chao1指数和Observed_species指数明显降低,Shannon指数升高,并可通过调节拟杆菌门、厚壁菌门、链霉菌属、乳酸杆菌属和弧菌属的丰度从而减轻小鼠肝脏的损伤程度。综上,VVFP可以减轻酒精引起的肝损伤,本研究可为多肽在功能食品领域的应用提供理论参考。

To investigate the preventive effect of Volvariella volvacea fruit body polypeptides(VVFP)on acute alcoholic liver injury in mice and its influence on the intestinal microbiota,VVFP(1–3 kDa molecular mass)which had been previously obtained by our laboratory was given by gavage to mice.The mice were randomly divided into six groups:blank control,model,positive control,low-dose VVFP,moderate-dose VVFP and high-dose VVFP.Serum indexes,liver indexes and histopathological sections were compared among these groups,and 16S rDNA gene high-throughput sequencing was used to analyze the diversity of the intestinal microflora and the relative abundance at the phyla and genus levels in each sample.Results showed that VVFP significantly reduced the levels of triglycerides(TG),total cholesterol(TC),alanine aminotransferase(ALT)and aspartate aminotransferase(AST)activity in the serum and malondialdehyde(MDA)in the liver,and decreased the levels of the inflammatory cytokines tumor necrosis factor-α(TNF-α)and interleukin-6(IL-6),and significantly increased the activities of alcohol dehydrogenase(ADH),total superoxide dismutase(T-SOD)and glutathione peroxidase(GSH-PX)in the liver.The 16S rRNA sequencing showed that VVFP significantly reduced theα-diversity indices Chao1 and observed species, increased the Shannon index, and regulated the abundance of Bacteroides, Firmicutes, Streptomyces, Lactobacillus and Vibrio, thereby reducing liver damage. In conclusion, VVFP can reduce alcoholic liver injury, which will provide a theoretical basis for the application of VVFP in the field of functional foods.