不同水稻品种OsVDAC8的表达模式比较与亚细胞定位分析

Comparison of Expression Patterns in Different Rice Varieties and Subcellular Localization Analysis of OsVDAC8

[目的]研究OsVDAC8的理化性质和表达模式,为探究OsVDAC8的功能提供基础。[方法]用生物信息学分析OsVDAC8的结构特征及表达谱,然后通过定量PCR对3个不同水稻品种日本晴(NIP)、粤泰A(YTA)、粤泰B(YTB)不同发育时期的不同组织中OsVDAC8的表达模式进行分析,并通过BiFC对亚细胞定位进行验证。[结果]OsVDAC8编码区全长1014 bp,编码337 aa,生物信息学分析结果显示,OsVDAC8是具有1个结构域的稳定的定位于线粒体的亲水蛋白,通过11次跨膜形成特异的桶状结构。在ATG上游含有13个与水稻生长发育及胁迫应答相关的顺式作用元件。RiceXPro和MSU Rice Genome Annotation Project水稻基因表达数据库分析结果表明,NIP中OsVDAC8在生殖生长期的茎和花序出现前后幼穗中表达水平最高,在胚乳和根中的表达量较低。对NIP、YTA、YTB 3个水稻品种中OsVDAC8的表达谱分析结果显示,OsVDAC8在3个水稻品种四叶期的叶鞘表达水平都非常高,在花粉内容物充实期都很低,但幼穗发育的不同时期3个品种中表达水平差异很大,NIP中幼穗发育的整个时期表达量比较稳定,YTB中表达量较低,而在YTA幼穗发育的中后期表达水平逐渐下降。亚细胞定位结果显示,OsVDAC8定位于线粒体,与预测结果一致。[结论]OsVDAC8在3个水稻品种中表达模式不同,YTB中整个幼穗发育过程中表达量较低,YTA幼穗发育的中后期表达水平逐渐下降,而NIP中幼穗发育的整个时期表达量都比较稳定。OsVDAC8定位于线粒体。

[Objective]To study the physical and chemical properties and expression patterns of OsVDAC8 for providing a basis for further investigation of the function of OsVDAC8.[Method]The structural characteristics and expression profile of OsVDAC8 were analyzed by bioinformatics.The expression patterns of OsVDAC8 in different tissues of three rice varieties,Nipponbare(NIP),Yuetai A(YTA)and Yuetai B(YTB)at different developmental stages were analyzed by quantitative PCR,and the subcellular localization was verified through BiFC.[Result]There were 1014 bp and encodes 337 aa in the OsVDAC8 coding region.The results of bioinformatics analysis showed that OsVDAC8 is a stable hydrophilic protein with one domain localized to mitochondria,and forms a specific barrel-like structure through 11 transmembrane.There are 13 cis-acting elements related to rice growth and development and stress response in the upstream of ATG.Analysis via rice XPro and MSU Rice Genome Annotation Project rice gene expression database showed that OsVDAC8 was expressed in the stem and panicle before and after the appearance of inflorescences during the reproductive growth period,and the expression was low in the endosperm and root in NIP.The expression profile analysis of OsVDAC8 in three rice varieties NIP,YTA and YTB showed that the leaf sheath expression level of OsVDAC8 was very high in the four-leaf stage of the three rice varieties and very low in the pollen content enrichment stage,but very different in the three rice varieties at different stages of young panicle development.In NIP,the expression level was relatively stable during the whole stage of young panicle development,while in YTB,the expression level was low,and in YTA,the expression level decreased gradually during the middle and late stage of young panicle development.The subcellular localization results showed that OsVDAC8 was localized to mitochondria,which was consistent with the prediction results.[Conclution]The expression patterns of OsVDAC8 were different in different rice varieties.OsVDAC8 was localized to mitochondria.