白背飞虱Tramtrack蛋白结构特点及在受精卵和5龄若虫头部的高表达

Structural Characteristics of Tramtrack Protein and Its High Expression in the Head of Zygotes and Fifth Instar Nymphs of Sogatella furcifera

[目的]了解重要的转录因子Tramtrack蛋白的结构特点,以及在卵和5龄若虫身体各部位的表达情况。[方法]在已获得的白背飞虱tramtrack基因的cDNA序列基础上,对其编码的Tramtrack蛋白结构特点进行生物信息学分析。[结果]该蛋白等电点为8.24,属于碱性蛋白,无信号肽,属于亲水性蛋白,有多个磷酸化位点,其三维结构主要是α-螺旋,少部分是β-折叠。定量PCR检测表明在白背飞虱受精卵中tramtrack基因的表达明显高于未受精卵;此外tramtrack基因在五龄若虫头部表达显著高于在胸部或腹部表达。[结论]这是第一次有关水稻害虫稻飞虱的Tramtrack蛋白的研究报道,为今后探究该蛋白在水稻害虫中的具体功能角色提供前期基础。

[Objective]To understand the structural characteristics of Tramtrack protein,and its expression in eggs and the 5th instar nymph.[Method]The structure of Tramtrack protein of Sogatella furcifera encoded by the obtained cDNA sequence of tramtrack gene was analyzed.[Result]The protein was a basic protein,no signal peptide,a hydrophilic protein with multiple phosphorylation sites and its isoelectric point was 8.24.The three-dimensional structure of the protein was mainlyα-helix,and a small part wasβ-fold.Quantitative PCR detected that the mRNA level of tramtrack gene in the fertilized eggs of white-backed planthopper was much higher compare to that in unfertilized eggs.In addition,the mRNA level of tramtrack gene in the head of fifth instar nymphs was much higher compare to that in the chest or abdomen.[Conclusion]This is the first detailed report on the Tramtrack protein of white-backed planthopper,which lays a foundation for further in vitro expression and functional study of this protein gene,and is conducive to the future exploration of the specific function and regulatory role of this protein in the growth and development of rice planthopper.