超声萃取测定海洋生物体中的石油烃

Determination of Petroleum Hydrocarbons in Marine Organisms by Ultrasonic Extraction

建立前处理超声萃取结合荧光分光光度法测定海洋生物体中的石油烃的分析方法。试样经氢氧化钠溶液和无水乙醇皂化后,用二氯甲烷萃取,萃取液过氧化铝柱去除肌肉组织干扰后,氮气吹至近干,环己烷定容,经荧光检测,计算出石油烃在生物体中的含量。结果表明:石油烃的方法检出限为0.17 mg·kg^(-1),在20.0~25.0 mg·kg^(-1)的质量分数范围下的加标回收率为90.8%~95%、相对标准偏差为4.4%~4.6%。该方法更便于操作,重复性较好,能够更好地满足实验需求,并归纳总结了此实验需注意的问题。

A method for the determination of petroleum hydrocarbons in marine organisms by pretreatment ultrasonic extraction combined with fluorescence spectrophotometry was established.After the sample was saponified with sodium hydroxide solution and anhydrous ethanol,it was extracted with dichloromethane.After the extraction liquid passed through the alumina column to remove the muscle tissue interference,nitrogen was used blown to the near dry,cyclohexane was used to fix volume,and the content of petroleum hydrocarbons in the organism was calculated by fluorescence detection.The results showed that the detection limit of petroleum hydrocarbon was 0.17 mg·kg^(-1),the recovery rate of spiked standard was 90.8%~95%in the content range of 20.0~25.0 mg·kg^(-1),and the relative standard deviation was 4.4%~4.6%.This method is more convenient to operate,has better repeatability,and can better meet the needs of the experiment.The problems that should be paid attention to in this experiment were summarized.