果王素抗大鼠肺纤维化的作用

Effects and mechanism of Kiwi fruit essence on pulmonary fibrosis in rats

目的 通过观察不同剂量的果王素对肺纤维化大鼠肺组织中成纤维细胞生长因子2(FGF2)及细胞外调节蛋白激酶1/2(ERK1/2)的影响。方法 SD大鼠分为空白对照组(0.9%NaCl),模型组(0.9%NaCl),阳性对照组(5 mg·kg^(-1)醋酸泼尼松),低、中、高剂量实验组(60、120、180 mg·kg^(-1)果王素),除空白对照组气管内一次性注入0.9%NaCl外,其余各组气管内一次性注入5 mg·kg^(-1)博来霉素A5构建大鼠肺纤维化模型。用蛋白质印迹法、实时荧光定量聚合酶链反应检测FGF2、ERK1/2蛋白及mRNA的表达情况。结果 空白对照、模型组、阳性对照组和高、中、低剂量实验组的FGF2蛋白相对表达水平在第28天时分别为0.07±0.01、0.63±0.15、0.14±0.02、0.16±0.01、0.26±0.02和0.44±0.03,ERK1/2蛋白相对表达水平在第28天时分别为0.06±0.00、0.41±0.05、0.09±0.00、0.10±0.00、0.14±0.02和0.21±0.02,FGF2 mRNA相对表达水平在第28天时分别为0.98±0.05、9.32±1.03、2.21±0.32、2.87±0.44、5.32±0.21和6.37±0.66,ERK1/2 mRNA在第28天相对表达水平分别为1.00±0.01、9.22±0.85、1.93±0.63、2.36±0.54、5.34±0.44和6.85±1.09。模型组的上述指标与空白对照组比较,在统计学上差异均有统计学意义(均P<0.05);阳性对照组和低、中、高剂量实验组的上述指标与模型组比较,在统计学上差异均有统计学意义(均P<0.05)。结论 果王素可能通过调控FGF2和ERK1/2的表达抑制肺纤维化。

Objective To observe the effects of different doses of Kiwi fruit essence on fibroblast growth factor 2(FGF2) and extracellular regulatory protein kinase 1/2(ERK1/2) in lung tissue of rats with pulmonary fibrosis.Methods SD rats were divided into blank control group(0.9% NaCl),model group(0.9% NaCl),positive control group(5 mg·kg^(-1 )prednesone acetate),experimental group-L,-M,-H(60,120,180 mg·kg^(-1 )Kiwi fruit essence),except the blank control group was injected with 0.9% NaCl at one time.The other groups were injected 5 mg·kg^(-1) bleomycin A5 into the trachea to construct the rat pulmonary fibrosis model.The expressions of FGF2 and ERK1/2 proteins and mRNA were detected by Western blot and real-time fluorescence quantitative polymerase chain reaction.Results The relative expression levels of FGF2 protein in blank control group,model group,positive control group and experimental group-L,-M,-H groups on the day 28 were 0.07±0.01,0.63±0.15,0.14±0.02 0.16 ± 0.01,0.26 ± 0.02 and 0.44 ± 0.03,respectively;the relative expression levels of ERK1/2 protein on day 28were 0.06 ± 0.00,0.41 ± 0.05,0.09 ± 0.00,0.10 ± 0.00,0.14 ± 0.02,0.21 ± 0.02,respectively;the relative expression levels of FGF2 mRNA on day 28 were 0.98 ± 0.05,9.32 ± 1.03,2.21 ± 0.32,2.87 ± 0.44,5.32 ± 0.21,6.37 ± 0.66,respectively;the relative expression levels of ERK1/2 mRNA on day 28 were1.00 ± 0.01,9.22 ± 0.85,1.93 ± 0.63,2.36 ± 0.54,5.34 ± 0.44 and 6.85 ± 1.09,respectively.The above indexes in the model group were significantly different from those in the blank control group( all P < 0.05).The above indexes in the positive control group and the experimental-L,-M,-H groups were significantly different from those in the model group( all P < 0.05).Conclusion Kiwi essence may inhibit pulmonary fibrosis by regulating the expression of FGF2 and ERK1/2.