多年生黑麦草VQ家族基因的鉴定及分析

Identification and expression analysis of VQ family genes in Lolium perenne L.

VQ(Valine-glutamine)是植物特有的一类蛋白,在植物生长发育及抵御生物与非生物胁迫中发挥着重要作用,但在多年生黑麦草(Lolium perenne L.)中鲜有VQ基因的相关研究报道。本研究利用生物信息学方法在多年生黑麦草基因组中鉴定了52个LpVQ基因,其不均匀地分布于7条染色体上。亚细胞定位结果显示,LpVQ蛋白主要定位于细胞核,仅LpVQ45定位于细胞膜。系统进化分析表明,LpVQ基因被分为7个亚家族,与拟南芥(Arabidopsis thaliana(L.)Heynh.)只存在4对直系同源基因。基因结构分析结果表明,46个LpVQ基因无内含子。荧光定量PCR分析结果显示,从各亚家族中随机选取的21个LpVQ基因不同程度地响应黑暗、高温及盐胁迫,且在胁迫早期快速响应。

Valine-glutamine(VQ)is a plant-specific protein,which plays an important role in plant growth,development,and resistance to biotic and abiotic stresses.To date,however,little research has been conducted on the VQ gene family in perennial ryegrass(Lolium perenne L.).In this study,52 LpVQ genes were identified in the perennial ryegrass genome,which were unevenly distributed on seven chromosomes.Subcellular localization prediction indicated that LpVQ proteins were mainly located in the nucleus,with only LpVQ45 located in the cell membrane.Phylogenetic analysis showed that LpVQ genes were divided into seven subfamilies and there were only four pairs of lineal homologous genes with Arabidopsis.Based on gene structure analysis of the LpVQ gene family,88.46%of LpVQ genes had no intron.Finally,three LpVQ genes were randomly from each subfamily to observe the responses to dark,high temperature,and salt stress.The qRT-PCR analysis LpVQ genes responded quickly in early stages of stress.This study provides a theoretical foundation for further study on VQ family genes of perennial ryegrass under abiotic stress.