铜藻多酚的体外抗炎和降血糖活性

In Vitro Anti-inflammatory and Hypoglycemic Activities of Polyphenols from Sargassum horneri

利用大孔吸附树脂纯化铜藻多酚粗提液,并研究纯化后铜藻多酚的体外抗炎和降血糖活性。利用脂多糖诱导RAW 264.7巨噬细胞建立体外炎症模型,结果发现,铜藻多酚降低炎症介质NO含量的最佳质量浓度为40μg/m L。与模型组相比,30μg/mL的铜藻多酚溶液可使白细胞介素(interleukin,IL)-6、肿瘤坏死因子α(tumor necrosis factor-α,TNF-α)和IL-1β 3种炎症因子的mRNA水平显著下调,对炎症因子TNF-α的蛋白表达水平有显著抑制作用,但是对炎症因子IL-1β蛋白表达水平抑制作用不显著,铜藻多酚对炎症因子的抑制作用与浓度呈正相关;铜藻多酚对α-葡萄糖苷酶的半数抑制浓度为5.96μg/mL,根据双倒数曲线发现铜藻多酚对α-葡萄糖苷酶的竞争性抑制常数Kic值为0.06μg/mL,非竞争性抑制常数Kiu值为6.68μg/mL,说明铜藻多酚对α-葡萄糖苷酶的抑制类型是非竞争性大于竞争性的混合可逆性抑制。以上结果表明铜藻多酚具有较好的抗炎和降血糖活性,可以作为一种天然的原料,应用于食品、保健品和化妆品等领域。

The crude polyphenols extracted from Sargassum horneri were purified using macroporous adsorption resin,and the in vitro anti-inflammatory and hypoglycemic activities of the purified product were studied.Lipopolysaccharide(LPS)was used to induce RAW 264.7 macrophages to establish an in vitro inflammatory model.The optimal mass concentration of polyphenols from S.horneri was 40μg/mL for reducing the NO content of inflammatory mediators.Compared with the model group,S.horneri polyphenols solution at 30μg/mL significantly down-regulated the mRNA expression levels of interleukin-6(IL-6),tumor necrosis factor-α(TNF-α)and IL-1β,and significantly inhibited the protein expression level of TNF-α,but had no significant inhibitory effect on the protein expression level of IL-1β;the inhibitory effect was positively correlated with the concentration of polyphenols.The half maximal inhibitory concentration(IC50)of S.horneri polyphenols onα-glucosidase was 5.96μg/mL.According to the double reciprocal curve,the competitive inhibition constant(Kic)and noncompetitive inhibition constant(Kiu)were 0.06 and 6.68μg/mL,respectively,indicating that the mode of inhibition was mixed-type with non-competitive being greater than competitive inhibition.Polyphenols from S.horneri have good anti-inflammatory and hypoglycemic activities,and can be used as a natural raw material in thefields of foods,health products and cosmetics.