3个李品种(系)芽需冷量的研究及休眠相关DAM基因的表达分析

Study on the Cold Demand of Buds of Three Plum Cultivars(Lines)and Expression Analysis of Dormancy-Related DAM Genes

【目的】探究3个李(Prunus)品种(系)的芽休眠和需冷量的差异及DAM基因表达与芽休眠的关系。【方法】调查统计了3个李品种(系)需冷量与萌芽率,采用生物信息学的方法对李的DAM基因进行鉴定,并用qRT-PCR对3个品种(系)李的DAM基因在芽的不同休眠阶段表达分析。【结果】7.2℃模型、0~7.2℃模型、犹他模型下“晚熟羌脆李”的需冷量均高于‘羌脆李’和‘脆红李’,且3个需冷量统计模型下‘羌脆李’和‘脆红李’的需冷量相同。鉴定了6个DAM基因(Pd DAM1、Pd DAM2、Pd DAM3、Pd DAM4、Pd DAM5、Pd DAM6),但花芽与叶芽内休眠期与内休眠解除期(需冷区间)高表达基因都为DAM5,且在休眠解除期DAM基因的相对表达量均呈下降趋势。【结论】揭示了3个李品种(系)的花芽与叶芽需冷量的差异,筛选出与休眠解除最相关的DAM基因,结果为李芽休眠及熟期调节提供理论依据。

【Objective】This study is to investigate the difference between bud dormancy and cold demand in three Prunus cultivars and the relationship between DAM gene expression and bud dormancy.【Method】The cold demand and germination rate of three plum cultivars(lines)were investigated,the DAM gene of plum was identified using bioinformatics method,and the expression of the DAM gene of the three varieties(lines)of plum was analyzed at different dormant stages of bud using qRT-PCR.【Result】The cooling requirements of"Wanshuqiangcuili"under the 7.2℃model,0~7.2℃model and Utah model were higher than those of′Qiangcuili′and′Cuihongli′,and the cooling requirements of′Qiangcuili′and′Cuihongli′under the three statistical models of cold demand were the same.Six DAM genes(PdDAM1,PdDAM2,PdDAM3,PdDAM4,PdDAM5,PdDAM6)were identified,but the high-expression genes in the dormancy period and the internal dormancy release period(cold interval)in flower buds and leaf buds was DAM5,and the relative expression of DAM genes decreased during the dormant release period.【Conclusion】The difference of cooling requirements between flower buds and leaf buds of the three plum cultivars(lines)was revealed,and the DAM gene most related to sleep release was screened.These results provided a theoretical basis for the regulation of plum bud dormancy and ripening period.