血清HDAC6和FoxO3a蛋白水平在预测妊娠期糖尿病并发重度子痫前期中的临床价值

Clinical value of serum histone deacetylase 6 and forkhead box O3a protein levels in predicting gestational diabetes mellitus complicated with severe preeclampsia

目的探究血清组蛋白去乙酰化酶6(HDAC6)和叉头框O3a(FoxO3a)蛋白水平在预测妊娠期糖尿病(GDM)并发重度子痫前期(sPE)中的临床价值。方法选取2021年7月至2022年7月在广元市第一人民医院分娩的224例单胎妊娠孕妇作为研究对象。其中GDM孕妇78例为GDM组,GDM并发sPE孕妇76例为GDM-sPE组,正常孕妇70例为对照组。采用酶联免疫吸附试验(ELISA)法检测血清HDAC6和FoxO3a的蛋白水平;采用Pearson法分析GDM-sPE患者血清HDAC6和FoxO3a的相关性;绘制受试者工作特征(ROC)曲线分析HDAC6和FoxO3a对GDM-sPE的预测价值。采用Logistic回归分析GDM-sPE发生的影响因素。结果GDM-sPE组HDAC6和FoxO3a水平均显著高于GDM组和对照组,GDM组显著高于对照组(P<0.05)。相关性分析显示,GDM-sPE患者血清HDAC6和FoxO3a的表达水平呈正相关(r=0.569,P<0.001)。ROC曲线分析显示,二者联合预测GDM-sPE发生的曲线下面积(AUC)高于HDAC6和FoxO3a单独预测的AUC(Z=24.214,P<0.001;Z=19.601,P<0.001)。Logistic回归分析显示,FBG、HDAC6和FoxO3a是GDM-sPE发生的影响因素(P<0.05)。结论GDM-sPE患者血清HDAC6和FoxO3a表达水平均显著升高,二者联合对GDM-sPE具有一定的预测价值。

Objective To explore the clinical value of serum histone deacetylase 6(HDAC6)and forkhead box O3a(FoxO3a)protein levels in predicting gestational diabetes mellitus(GDM)complicated with severe preeclampsia(sPE).Methods A total of 224 singleton pregnancy women who gave birth in the First People′s Hospital of Guangyuan from July 2021 to July 2022 were selected as the study objects.Among them,78 GDM pregnant women were set as GDM group,76 GDM complicated with sPE pregnant women were set as GDM-sPE group,and 70 normal pregnant women were set as control group.Enzyme-linked immunosorbent assay(ELISA)was applied to detect the serum protein levels of HDAC6 and FoxO3a.Pearson analysis was applied to analyze the correlation between serum HDAC6 and FoxO3a in GDM-sPE patients.Receiver operating characteristic(ROC)curve was drawn to analyze the predictive value of HDAC6 and FoxO3a on GDM-sPE.Logistic regression analysis was applied to analyze the influencing factors of GDM-sPE occurence.Results The levels of HDAC6 and FoxO3a in GDM-sPE group were significantly higher than those in GDM group and control group,and those in GDM group were significantly higher than those in control group(P<0.05).Correlation analysis showed that the serum expression levels of HDAC6 and FoxO3a in GDM-sPE patients was positively correlated(r=0.569,P<0.001).The ROC curve analysis showed that the area under curve(AUC)value predicted by HDAC6 and FoxO3a combined was higher than HDAC6 and FoxO3a alone(Z=24.214,P<0.001;Z=19.601,P<0.001).Logistic regression analysis showed that FBG,HDAC6 and FoxO3a were the influencing factors of GDM-sPE occurrence(P<0.05).Conclusions The serum expression levels of HDAC6 and FoxO3a in patients with GDM-sPE are significantly increased,and the combination of them has a certain predictive value for GDM-sPE.