GARP、Foxp3及TGF-β在桥本甲状腺炎病人PBMC中的表达及意义

Expression and significance of GARP,Foxp3 and TGF-βin PBMC of Hashimoto′s thyroiditis patients

目的:探讨桥本甲状腺炎(HT)病人外周血单个核细胞(PBMC)中糖蛋白A重复序列为主的蛋白(GARP)、叉头框蛋白p3(Foxp3)、转化生长因子β(TGF-β)mRNA表达水平和意义。方法:选取HT病人32例作为观察组,选取同期健康体检者32名作为对照组。采用化学发光免疫法测定2组血清促甲状腺激素(TSH)、总三碘甲状腺原氨酸(TT3)、总甲状腺素(TT4)水平,放射免疫法检测甲状腺抗体TmAb、TgAb水平。采用实时荧光定量PCR检查HT病人PBMC中GARP、Foxp3、TGF-βmRNA,分析HT病人PBMC中GARP、Foxp3、TGF-βmRNA表达量与甲状腺功能指标水平的相关性。结果:2组TT3、TT4、TSH水平差异均无统计学意义(P>0.05),观察组TgAb和TmAb水平均明显高于对照组(P<0.01)。观察组GARP、Foxp3、TGF-βmRNA表达水平均明显低于对照组(P<0.01)。Pearson相关分析显示,HT病人PBMC中GARP mRNA表达水平与TgAb水平呈负相关关系(P<0.05),与TT3水平呈明显正相关关系(P<0.01)。结论:HT病人PBMC中GARP、Foxp3、TGF-βmRNA表达降低。GARP mRNA与TgAb水平呈负相关关系,与TT3水平呈明显正相关关系,可能与HT的发生、发展相关。

Objective:To investigate the expression level of mRNA and significance of glycoprotein-A repetitions predominant protein(GARP),Forkhead box protein p3(Foxp3)and transforming growth factorβ(TGF-β)in peripheral blood mononuclear cells(PBMC)of Hashimoto′s thyroiditis(HT)patients.Methods:Thirty-two patients with HT were selected as the observation group and 32 healthy examinees as the control group.The levels of serum thyroid stimulating hormone(TSH),total triiodothyronine(TT3)and total thyroxine(TT4)were measured by chemiluminescence immunoassay,and the levels of thyroid antibodies TmAb and TgAb were detected by radioimmunoassay.Real-time fluorescent quantitative PCR was used to detect the expression levels of GARP,Foxp3 and TGF-βmRNA in PBMC of HT patients.The correlation of GARP,Foxp3 and TGF-βmRNA expression level in PBMC of HT patients with the level of thyroid function index was analyzed.Results:There was no significant difference in TT3,TT4 and TSH levels between the two groups(P>0.05).The levels of TgAb and TmAb in the observation group were significantly higher than those in the control group(P<0.01).The expression levels of GARP,Foxp3 and TGF-βmRNA in the observation group were significantly lower than those in the control group(P<0.01).Pearson correlation analysis showed that the expression level of GARP mRNA in PBMC of HT patients was negatively correlated with the level of TgAb(P<0.05)and significantly positively correlated with the level of TT3(P<0.01).Conclusions:The expression of GARP,Foxp3 and TGF-βmRNA in PBMC of HT patients is decreased.GARP mRNA is negatively correlated with the level of TgAb and significantly positively correlated with the level of TT3,which may be related to the occurrence and development of HT.