SLC35A2、前叶黄素亚基2在乳腺癌中的表达及其与临床观察指标和预后的关系

Expression of SLC35A2 and PFDN2 in breast cancer and its relationship with clinical observables and prognosis

目的研究SLC35A2、前叶黄素亚基2(PFDN2)在乳腺癌中的表达及其与临床观察指标和预后的关系。方法使用TCGA数据库及TIMER 2.0数据库分析SLC35A2、PFDN2在乳腺癌组织及癌旁组织中的表达差异;K-M数据库绘制两者高、低表达组患者的生存曲线。qRT-PCR及免疫组化法检测SLC35A2、PFDN2在癌和癌旁组织的表达,统计学分析两者在两组织中的表达差异及其表达水平与临床观察指标之间的关系,并筛选出乳腺癌独立预后因素;将两者按表达水平分组,使用K-M生存分析比较各组预后差异并绘制生存曲线。结果生信、qRT-PCR及免疫组化结果显示SLC35A2、PFDN2在乳腺癌癌组织中表达水平显著高于癌旁组织。SLC35A2的表达与淋巴结转移情况显著相关,PFDN2的表达与肿瘤直径及淋巴结转移情况显著相关,两者高表达是乳腺癌的独立预后因素,两者均为高表达的患者预后最差。结论乳腺癌组织中SLC35A2和PFDN2的表达与临床观察指标和乳腺癌预后密切相关,可作为乳腺癌治疗的潜在靶点。

Objective To investigate the expression of SLC35A2 and PFDN2 in breast cancer and their relationship with clinical indicators and prognosis.Methods TCGA database and TIMER 2.0 database were used to analyze the differences of SLC35A2 and PFDN2 expression in breast cancer tissues and paracancerous tissues;K⁃M database was used to create the survival curves of patients in the high and low expression groups of the two.qRT⁃PCR and immunohistochemistry were used to detect the expression of SLC35A2 and PFDN2 in the cancerous and paracancerous tissues,and the expression differences,the relationship between their expression levels and the clinical observation indexes were statistically analyzed,and the independent prognostic factors of breast cancer were screened out;K⁃M survival analysis was used to compare the prognostic differences between the groups and create the survival curves.Results The expression levels of SLC35A2 and PFDN2 in breast cancer tissues were significantly higher than those in paracancerous tissues according to the results of biopsy,qRT⁃PCR and immuno⁃histochemistry,and the expression levels of SLC35A2 were significantly correlated with lymph node metastasis,while the expression of PFDN2 was significantly correlated with the diameter of the tumor and the metastasis of lymph nodes,and the expression of SLC35A2 and PFDN2 was an independent prognostic factor for breast cancer.patients had the worst prognosis.Conclusion The expression of SLC35A2 and PFDN2 in breast cancer tissues was closely related to clinical indicators and prognosis of breast cancer,and could be used as a potential target for breast cancer treatment.