WD重复域蛋白5在宫颈癌组织中的表达及其与患者临床病理特征的关系

The expression of WDR5 in cervical cancer tissue and its relationship with clinical and pathological characteristics of patients

目的探讨WD重复域蛋白5(WD repeat-containing protein 5,WDR5)蛋白在宫颈癌(cervical cancer,CA)组织中的表达及其与患者临床病理特征的关系。方法纳入2018年1月至2020年3月山东第一医科大学附属人民医院接收的CA患者(105例)为研究对象,收集患者的癌组织及癌旁组织标本,采用免疫组织化学染色法及Western blot法检测CA组织及癌旁组织中WDR5水平采用免疫组化以及Western blot法测定;生存分析采用Kaplan-Meier法;患者预后影响因素通过Cox回归进行分析。结果105例CA组织标本中,WDR5的阳性表达率(WDR5阳性例数/癌组织总例数)为68.57%(72/105)高于癌旁组织22.86%(24/105)(P<0.05);相较于癌旁组织(1.00±0.11),WDR5的表达量在CA组织(4.66±0.98)中较高(t=38.030,P<0.05);WDR5表达量与分化程度、TNM分期、淋巴结转移有关(P<0.05);WDR5阳性表达的生存率65.28%(47/72)低于阴性表达90.91%(30/33)(Log rankχ2=6.732,P=0.009);TNM分期、WDR5、分化程度、淋巴结转移均是患者预后的影响因素(P<0.05)。结论WDR5表达在CA患者癌组织中升高,其水平变化与CA患者TNM分期、分化程度、淋巴结转移及预后密切相关。

Objective To investigate the expression of WD repeat-containing protein 5(WDR5)in cervical cancer tissue and its relationship with clinical pathological characteristics and prognosis of patients.Methods 105 CA patients admitted to our hospital from January 2018 to March 2020 were included as the study subjects,the cancer tissue and adjacent tissue samples of patients were collected,Immunohistochemical staining and Western blot were used to detect the level of WDR5 in CA tissue and adjacent cancer tissues.Immunohistochemistry and Western blot were used to determine the level;Survival analysis was conducted using the Kaplan Meier method;The influencing factors of patient prognosis were analyzed through Cox regression.Results Among 105 CA tissue samples,the positive expression rate of WDR5(WDR5 positive cases/total cancer tissue cases)was 68.57%(72/105),which was higher than 22.86%(24/105)in adjacent cancer tissues(P<0.05);Compared to adjacent tissues(1.00±0.11),the expression level of WDR5 was higher in CA tissues(4.66±0.98)(t=38.030,P<0.05).The expression level of WDR5 is related to the degree of differentiation,TNM staging,and lymph node metastasis(P<0.05);The survival rate of WDR5 positive expression was 65.28%(47/72)lower than that of negative expression of 90.91%(30/33)(Log rankχ2=6.732,P=0.009);TNM staging,WDR5,degree of dif⁃ferentiation,and lymph node metastasis are all influencing factors for patient prognosis(P<0.05).Conclusion The expression of WDR5 is elevated in cervical cancer tissues,and its changes are closely related to TNM staging,differentiation,lymph node metastasis,and prognosis in cervical cancer patients.