富血小板血浆联合玻璃酸钠对骨质疏松性骨折大鼠的影响

Effect of platelet-rich plasma combined with sodium hyaluronate on osteoporotic fracture in rats

目的探讨富血小板血浆联合玻璃酸钠对骨质疏松性骨折大鼠骨愈合、血管生成及NFATc1信号通路的影响。方法选取50只SPF级SD雌性大鼠,随机分为正常(NO)组,模型(MO)组,富血小板血浆(PP)组,玻璃酸钠(SH)组,富血小板血浆联合玻璃酸钠(PS)组。通过小动物骨密度仪检测骨密度、生物力学试验机检测骨生物力学、HE染色法检测骨组织病理形态、ELISA法检测血清中血管生成相关因子含量、免疫印迹法检测大鼠骨组织中NFATc1信号通路蛋白表达。结果与NO组相比,MO组骨密度、刚度、最大载荷、最大压强、弹性模量、血管数量及血管面积、VEGF、bFGF、ANG-1含量显著降低(P<0.05),股骨组织NFATc1、OSCAR蛋白表达显著升高(P<0.05),与MO组相比,PP组、SH组、PS组骨密度、刚度、最大载荷、最大压强、弹性模量、血管数量及血管面积、VEGF、bFGF、ANG-1含量明显升高(P<0.05),股骨组织NFATc1、OSCAR蛋白表达显著降低(P<0.05),且SH组与PP组相比无明显差异(P>0.05),PS组比SH组变化显著(P<0.05)。结论富血小板血浆联合玻璃酸钠对骨质疏松性骨折大鼠具有显著疗效,可有效促进骨愈合及血管生成,并抑制NFATc1信号通路。

Objective To explore the effects of platelet-rich plasma combined with sodium hyaluronate on bone healing,angiogenesis and NFATc1 signaling pathway in rats with osteoporotic fractures.Methods Fifty SPF SD female rats were randomly divided into normal(NO)group,model(MO)group,platelet-rich plasma(PP)group,sodium hyaluronate(SH)group,and platelet-rich plasma combined with sodium hyaluronate(PS)group.Bone mineral density was detected by bone densitometry in small animals,bone biomechanics was detected by biomechanics testing machine,bone tissue pathological morphology was detected by HE staining,serum angiogenesis related factors were detected by ELISA,and NFATc1 signaling pathway protein expression in rat bone tissue was detected by western blot.Results Compared with NO group,bone mineral density,stiffness,maximum load,maximum pressure,elastic modulus,number and area of blood vessels,VEGF,bFGF and ANG-1 contents in MO were significantly decreased(P<0.05),while the expressions of NFATc1 and OSCAR protein in femur tissue were significantly increased(P<0.05).Bone mineral density,stiffness,maximum load,maximum pressure,elastic modulus,blood vessel number and blood vessel area,VEGF,bFGF and ANG-1 contents in PP,SH and PS groups were significantly increased(P<0.05),while the expressions of NFATc1 and OSCAR proteins in femur tissue were significantly decreased(P<0.05).There was no significant difference between SH group and PP group(P>0.05),and PS group had significant change compared with SH group(P<0.05).Conclusion Platelet-rich plasma combined with sodium hyaluronate has a significant effect on osteoporotic fracture rats,which can effectively promote bone healing and angiogenesis,and inhibit NFATc1 signaling pathway.