壮骨强肌方对去势大鼠骨密度及IL-17的影响

Zhuanggu Qiangji Formula on bone mineral density and IL-17 level in ovariectomized rats

目的探讨壮骨强肌方对去卵巢大鼠骨密度及IL-17的影响。方法30只SPF级6月龄雌性SD大鼠按随机数字表分为Sham组(假手术组,n=9)和模型组(n=21),模型组予以双侧输卵管结扎并卵巢切除,Sham组切除卵巢周围等体积脂肪组织,12周后两组各随机抽取3只大鼠检测腰椎骨密度验证造模。造模成功后,将模型组大鼠按照随机数字表分为OVX组(模型对照组)、ZGQJ组(壮骨强肌方组)和Alen组(阿仑膦酸钠组),每组6只。ZGQJ组予以壮骨强肌方,Alen组予以阿仑膦酸钠,OVX组和Sham组大鼠予以等体积生理盐水,均灌胃给药,连续给药12周后取材。DEXA检测腰椎骨密度、Micro-CT检测胫骨骨小梁微结构、股骨HE染色、血清IL-17检测、胫骨IL-17免疫荧光染色,脾脏HE及IL-17免疫组化染色。结果与OVX组比较,ZGQJ组与Alen组腰椎BMD增高(P<0.05),ZGQJ组与Alen组胫骨BV/TV、Tb.N及Tb.Th增高(P<0.05)且Tb.Sp降低(P<0.05),ZGQJ组与Alen组股骨HE染色骨小梁面积增多(P<0.05),ZGQJ组与Alen组血清Elisa检测IL-17水平降低(P<0.05),ZGQJ组胫骨免疫荧光染色IL-17表达降低(P<0.05),ZGQJ组脾脏HE染色组织病理结构改善,ZGQJ组脾脏指数提高(P<0.05),ZGQJ组脾脏免疫组化染色IL-17表达降低(P<0.05)。结论中药壮骨强肌方具有提高去卵巢大鼠腰椎骨密度、改善胫骨及股骨骨小梁结构、降低IL-17表达及改善脾脏免疫功能的作用。

Objective To investigate the effect of the strong bone and muscle formula on bone mineral density(BMD)and IL-17 level in ovariectomized rats.Methods Thirty SPF grade 6-month-old female SD rats were divided into Sham group(n=9)and model group(n=21)according to random number table.After 12 weeks,3 rats were respectively selected from the Sham group and OVX group for lumbar vertebrae BMD test to verify the success of modeling.Rats in the model group were divided into OVX group(n=6),ZGQJ group(n=6),and Alen group(n=6).Rats in ZGQJ group received the strong bone and muscle formula.Rats in Alen group received alendronate sodium.After 12 weeks of the treatment,the rats were sacrificed.BMD of the lumbar vertebrae was detected with DEXA.The microstructure of the tibia was detected with micro-CT.The structure of the femur and spleen was observed with HE staining.Serum level of IL-17 was detected with ELISA.The level of IL-17 in the tibia was detected with immunofluorescent staining.The level of IL-17 in the spleen was detected with immunohistochemistry.Results Compared to that in OVX group,the lumbar vertebrae BMD in ZGQJ and Alen group increased significantly(P<0.05),BV/TV,Tb.N,and Tb.Th in ZGQJ and Alen groups increased significantly(P<0.05),but Tb.Sp decreased significantly(P<0.05).Compared to that in OVX group,trabecular area of the femur in ZGQJ and Alen group increased(P<0.05),serum level of IL-17 in ZGQJ and Alen group decreased(P<0.05),and the expression of IL-17 in the tibia of ZGQJ group decreased(P<0.05).The histopathologic structure of the spleen in ZGQJ group improved,the spleen index in ZGQJ group improved,and the expression of IL-17 in the spleen in ZGQJ group decreased(P<0.05).Conclusion The strong bone and muscle formula improves BMD of the lumbar vertebrae,improves trabecular structure of the tibia and femur,reduces the expression of IL-17,and improves immune function of the spleen in OVX rats.