HPLC多指标成分定量联合化学计量学评价不同产地茜草

Evaluation of Rubiae Radix from different regions by HPLC multi index components combined with chemometrics

目的 建立茜草多指标成分定量联合化学计量学评价不同产地茜草的质量。方法 采用高效液相色谱法测定8省区18批茜草中新橙皮苷、山柰酚、茜草素、异茜草素、羟基茜草素、1,3,6-三羟基-2-甲基蒽醌、大叶茜草素、去氢-α-拉杷醌、β-谷甾醇和异落叶松脂素,并采用主成分分析、正交偏最小二乘法-判别分析、灰色关联度分析对测定结果进行评价。结果相同产区的茜草,质量较接近;共找到4个差异性标志成分,其影响显著性排序为大叶茜草素>茜草素>1,3,6-三羟基-2-甲基蒽醌>羟基茜草素;陕西、河南和安徽所产的茜草整体质量较好。结论 方法便捷、准确,易于推广,可以更全面、更系统地评价茜草的质量。

Objective To establish a method for quality evaluation of Rubiae Radix by HPLC combined with chemometrics.Methods The contents of neohesperidin,kaempferol,alizarin,xanthopurpurin,purpurin,1,3,6-trihydroxy-2-methylanthraquinone,mollugin,dehydro-α-lapachone,β-sitosterol,and isolariciresinol in 18 batches of Rubiae Radix collected from 8 provinces were determined by HPLC method,and the results were analyzed by principal component analysis(PCA),orthogonal partial least squares discriminant analysis(OPLS-DA),and grey relational analysis(GRA)method.Results Rubiae Radix from the same production area had similar quality.A total of four differential marker components were found,and their significance was ranked as follows:mollugin>alizarin>1,3,6-trihydroxy-2-methylanthraquinone>purpurin.The overall quality of Rubiae Radix in Shaanxi,Henan,and Anhui Province was good.Conclusion The method is convenient,accurate,and easy to promote,which can comprehensively and systematically evaluate the quality of Rubiae Radix.