高糖环境下XIST对人视网膜血管内皮细胞凋亡的影响

Effects of XIST on apoptosis of human retinal vascular endothelial cells under high glucose environment

目的:探讨高糖环境下长链非编码RNA X-非活性特异转录本(XIST)对人视网膜血管内皮细胞凋亡的影响及可能机制。方法:以人视网膜血管内皮细胞为研究对象,设对照组和高糖组,分析高糖对人视网膜血管内皮细胞XIST表达的影响;设对照组、高糖组、高糖+XIST组、高糖+阴性对照组,分析过表达XIST对高糖诱导人视网膜血管内皮细胞增殖和凋亡的影响;设对照组、高糖组、高糖+XIST组、高糖+XIST+pcDNA3.1-Wnt1组、高糖+XIST+pcDNA3.1-CON组,分析XIST通过Wnt1/β-catenin通路对高糖诱导人视网膜血管内皮细胞增殖和凋亡的影响。结果:(1)高糖组XIST相对表达量较对照组低(P<0.05)。(2)与对照组比,高糖组细胞增殖活力低,而凋亡率、Wnt1和β-catenin水平高(P<0.05);与高糖+阴性对照组比,高糖+XIST组增殖活力高,而凋亡率、Wnt1和β-catenin水平低(P<0.05)。(3)与对照组比,高糖组细胞增殖活力低,而凋亡率高(P<0.05);与高糖组比,高糖+XIST组增殖活力高,而凋亡率低(P<0.05);与高糖+XIST+pcDNA3.1-CON组比,高糖+XIST+pcDNA3.1-Wnt1组增殖活力低,而凋亡率高(P<0.05)。结论:XIST通过负调控Wnt1/β-catenin通路抑制高糖诱导的人视网膜血管内皮细胞凋亡。

Objective:To investigate the effect and possible mechanism of long non-coding RNA X-inactive specific transcript(XIST)on apoptosis of human retinal vascular endothelial cells under high glucose environment.Methods:Taking human retinal vascular endothelial cells as the research objects,firstly to analyze the effect of high glucose on the expression of XIST in human retinal vascular endothelial cells,the cells were divided into control group and high glucose group.Then to analyze the effect of overexpression of XIST on the proliferation and apoptosis of human retinal vascular endothelial cells induced by high glucose,the cells were divided into control group,high glucose group,high glucose+XIST group,and high glucosenegative control group.Finally in order to analyze the effect of XIST on the proliferation and apoptosis of human retinal vascular endothelial cells induced by high glucose through the Wnt1/β-catenin pathway,the cells were divided into control group,high glucose group,high glucose+XIST group,high glucose+XIST+pcDNA3.1-Wnt1 group,high glucose+XIST+pcDNA3.1-CON group.Results:(1)The relative expression of XIST in the high glucose group was lower than that in the control group(P<0.05).(2)Compared with the control group,the cell proliferation activity in the high glucose group was lower,while the apoptosis rate,Wnt1 andβ-catenin protein expression levels were higher(P<0.05).The+XIST group had higher cell proliferation activity,while the apoptosis rate,Wnt1 andβ-catenin protein expression levels were lower(P<0.05).(3)Compared with the control group,the cell proliferation activity in the high glucose group was lower,but the apoptosis rate was higher(P<0.05).Compared with the high glucose group,the cell proliferation activity in the high glucose+XIST group was higher,and the apoptosis rate was lower.Compared with the high glucose+XIST+pcDNA3.1-CON group,the high glucose+XIST+pcDNA3.1-Wnt1 group had lower cell proliferation activity and higher apoptosis rate(P<0.05).Conclusion:XIST inhibits high glucose-induced apoptosis of human retinal vascular endothelial cells by negatively regulating the Wnt1/β-catenin pathway.