酵母强化大曲接种量优化及其生产应用

Optimization of inoculation amount and its production application of yeast-fortified Daqu

为提高泥坑浓香型白酒中高温大曲综合生产性能,该研究从中高温大曲中分离筛选菌株,采用2,3,5-氯化三苯基四氮唑(TCC)初筛,发酵实验复筛,获得发酵性能良好的酵母菌,并以Z综合得分为响应值,通过单因素试验及响应面试验对筛选菌株接种量进行优化,并将其用于强化大曲生产。结果表明,共分离纯化出8株菌株,其中,3株产酒性能良好的菌株Y1、Y2、Y3,其最佳接种量分别为7.0×10^(8)CFU/mL、3.0×10^(8)CFU/mL、1.5×10^(8)CFU/mL,在此优化复配条件下,主成分Z综合得分为513,所生产的强化大曲发酵力、糖化力和液化力分别为3.46 g/(g·72 h)、994 mg/(g·h)、1.12 g/(g·h),比优化前提高了424%、6.7%和5.7%。

In order to improve the comprehensive production performance of medium-high-temperature Daqu in strong-flavor(nongxiangxing)Baijiu,strains were separated and screened from medium-high-temperature Daqu,and 2,3,5-triphenyltetrazolium chloride(TCC)was used for preliminary screening,and the fermentation experiments were used for rescreening to obtain yeast with good fermentation performance.The Z comprehensive score was used as the response value.Through single factor and response surface test,the inoculation of screened strains was optimized,and it was used to strengthen Daqu production.The results showed that a total of 8 strains were isolated and purified,among which 3 strains of yeast Y1,Y2,and Y3 with good alcohol production performance were screened out.The inoculum of 3 strains of yeast was 7.0×10^(8) CFU/ml,3.0×10^(8) CFU/ml,1.5×10^(8) CFU/ml.Under the optimized combination conditions,the overall score of principal component Z was 513.The fermentation power,saccharification power and liquefaction power were 3.46 g/(g·72 h),994 mg/(g·h)and 1.12 g/(g·h),respectively,which were increased by 424%,6.7%and 5.7%compared with before optimization.