IL22基因多态性与口腔扁平苔藓易感性及唾液IL-22水平升高的关联

Association of IL22 gene polymorphism with susceptibility to oral lichen planus and elevated salivary IL-22 levels

目的:探讨白细胞介素22(interleukin 22,IL22)基因位点(rs1026788、rs1179249及rs2227472)单核苷酸多态性(single nucleotide polymorphisms,SNPs)与口腔扁平苔藓(oral lichen planus,OLP)易感性和唾液IL-22水平的关联。方法:选取2020年1月~2022年6月我院口腔门诊收治的经病理诊断为OLP患者的全血标本174份,同期选取无口腔黏膜疾病史的健康志愿者血液标本206份,比较两组IL22基因位点的SNPs分布频率,以及与OLP易感风险及唾液IL-22水平的相关性。结果:IL22基因rs1026788、rs1179249及rs2227472位点的基因型频率在两组中的分布均符合Hardy-Weinberg遗传平衡定律。与祖先型等位基因和基因型相比,rs1026788位点或rs2227472位点的突变等位基因G和基因型GG与OLP易感性增加显著相关,然而rs1179249位点的突变等位基因A和基因型AA与OLP易感性降低相关(P<0.05)。通过Cochran-Armitage趋势检验,OLP的风险随着突变数量的增加而增加(P=0.01)。至少携带rs1026788、rs1179249、rs222742等位基因之一的受试者OLP易感性增加(OR=2.096,P=0.016)。携带rs1179249 AC/AA基因型的OLP患者唾液IL-22水平略低于CC基因型(P=0.024)。结论:IL22基因位点rs1026788、rs1179249及rs2227472可能是中国汉族人群OLP易感性的遗传标记;而且rs1179249可能影响唾液IL-22水平。

Objective:To investigate the association between single nucleotide polymorphisms(SNPs)at the interleukin-22(IL22)gene loci(rs1026788,rs1179249,and rs2227472)and susceptibility to oral lichen planus(OLP)and salivary IL-22 levels.Methods:Totally 174 whole blood samples from patients with OLP diagnosed by pathology admitted to our dental clinic and 206 blood samples from healthy volunteers without a history of oral mucosal diseases from January 2020 to June 2022 were selected.The SNPs distribution frequency of the IL22 gene locus and its correlation with susceptibility to OLP and saliva IL-22 levels were compared between the two groups.Results:The distribution of genotype frequencies at the rs1026788,rs1179249,and rs2227472 loci of the IL22 gene in both groups was consistent with Hardy-Weinberg's law of genetic balance.Compared with the ancestral alleles and genotypes,the mutated allele G and genotype GG at rs1026788 or rs2227472 were significantly associated with increased susceptibility to OLP,while the mutated allele A and genotype AA at rs1179249 were associated with decreased susceptibility to OLP(P<0.05).Through the Cochran-Armitage trend test,the risk of OLP increased with the number of mutations(P=0.01).Subjects carrying at least one of the alleles rs1026788,rs1179249,and rs222742 had an increased susceptibility to OLP(OR=2.096,P=0.016).The saliva IL-22 levels in OLP patients carrying the rs1179249 AC/AA genotype were slightly lower than those of the CC genotype(P=0.024).Conclusion:The IL22 loci rs1026788,rs1179249 and rs2227472 may be the genetic marker of OLP susceptibility in Chinese Han population.Moreover,salivary IL-22 levels may be affected by rs1179249.