贝莱斯芽孢杆菌BQ对党参根腐病病原菌抑制效应初探

Evaluation on the Control Effects of Bacillus velezensis BQ against Codonopsis pilosula Root Rot

将贝莱斯芽孢杆菌(Bacillus velezensis)BQ作为最新发现的潜力生防菌。通过平板对峙、孢子萌发、生防机制和离体防治试验检测该菌株对党参根腐病的防治效果。平板对峙试验表明,贝莱斯芽孢杆菌BQ可明显抑制党参根腐病病原菌尖孢镰孢菌DS的生长,菌丝生长抑制率达50.55%,对峙区菌丝发生明显形变;孢子萌发结果表示,贝莱斯芽孢杆菌BQ的代谢产物能有效抑制病原菌DS的孢子萌发;对贝莱斯芽孢杆菌的生防机制进行初探,贝莱斯芽孢杆菌BQ的代谢产物能引起病原菌DS的细胞膜通透性增加,从而导致核酸内容物释放量增加,细胞破裂至死亡。离体防治结果显示,贝莱斯芽孢杆菌BQ对党参根腐病的治疗效果达到46.13%,预防效果达到了63.62%。可见,贝莱斯芽孢杆菌BQ有防治党参根腐病的潜力,可作为生物防治剂进行开发。

Bacillus velezensis BQ was regarded as the newly discovered potential biocontrol bacteria,and its control effects on root disease of Codonopsis pilosula was studied by the dual confrontation test,spore germination,biological control mechanism and in vitro control test.The results of the dual confrontation test showed that Bacillus velezensis BQ could obviously inhibit the growth of Fusarium oxysporum DS,the mycelium growth inhibition rate reached 50.55%,and the mycelium in the confrontation area was obviously deformed.The results of spores germination showed that the metabolites of Bacillus velezensis BQ could effectively inhibit the spores germination of pathogen DS.The metabolites of Bacillus velezensis BQ could increase the permeability of cell membrane of pathogenic bacteria DS,which led to the increase of nucleic acid release and cell rupture to death.In vitro control results showed that Bacillus velezensis BQ had a therapeutic effect of 46.13%and a preventive effect of 63.62%on Codonopsis pilosula root rot.Bacillus velezensis BQ has the potential to control Codonopsis pilosula root rot and can be developed as a biological control agent.