慢性乙型肝炎患者血清FGF-9、S100A9水平及其与病情严重程度的相关性

The levels of serum FGF-9 and S100A9 in patients with chronic hepatitis B and their correlation with the severity of the dis-ease

目的探究慢性乙型肝炎(CHB)患者血清成纤维细胞生长因子-9(FGF-9)、S100A9水平与病情严重程度的相关性.方法选取2021年5月-2022年5月我院收治的118例CHB患者为CHB组,同期选择在我院体检的健康体检者118例即为对照组.采用酶联免疫吸附测定(ELISA)法检测血清中FGF-9、S100A9表达水平;采用实时荧光定量PCR(qRT-PCR)法检测HBV-DNA水平;采用Pearson法分析CHB患者血清FGF-9、S100A9水平与HBV-DNA水平的相关性;经过Spearman法分析CHB患者FGF-9、S100A9、HBV-DNA水平与炎症程度、肝纤维化分期的相关性;采用多因素Logistic分析中重度CHB发生的影响因素.结果与对照组相比,CHB患者血清中FGF-9、S100A9水平显著升高(P<0.05).与轻度组相比,中度组和重度组患者血清中FGF-9、S100A9水平依次显著升高(P<0.05).随着炎症程度的不断增加,CHB患者血清中FGF-9、S100A9水平呈逐渐上升的趋势(P<0.05).随着肝纤维化分期的逐渐增加,CHB患者血清中FGF-9、S100A9水平呈现逐渐增加的趋势(P<0.05).CHB患者血清中FGF-9、S100A9水平与HBV-DNA水平呈正相关关系(P<0.05).CHB患者FGF-9、S100A9、HBV-DNA水平与炎症程度、肝纤维化分期均呈正相关(P<0.05).FGF-9水平、S100A9水平均是发生中重度CHB的独立危险因素(P<0.05).结论CHB患者血清中FGF-9、S100A9与病情严重程度相关,能够作为评估CHB患者病情程度的参考指标.

Objective To explore the correlation between the serum levels of fibroblast growth factor 9(FGF-9)and S100A9 and the severity of chronic hepatitis B(CHB).Methods A total of 118 CHB patients admitted to our hospital from May 2021 to May 2022 were regarded as the CHB group,while 118 healthy individuals who underwent physical examination in our hospital were regarded as the control group during the same period.ELISA(ELISA)was applied to detect the expression levels of FGF-9 and S100A9 in ser-um;real-time fluorescence quantitative PCR(qRT-PCR)method was applied to detect HBV-DNA levels;Pearson method was applied to analyze the correlation between serum FGF-9,S100A9 levels and HBV-DNA level in CHB patients;Correlation between FGF-9,S100A9,and HBV-DNA levels,inflammation degree,and liver fibrosis stage in CHB patients analyzed by Spearman method;Using Multivariate Logistic Analysis to Analyze the Influencing Factors of Moderate to Severe CHB.Results Compared with the control group,the levels of FGF-9 and S100A9 in the serum of CHB patients were obviously higher(P<0.05).Compared with the mild group,the serum levels of FGF-9 and S100A9 in the moderate and severe groups were obviously higher(P<0.05).As the degree of inflammation continued to increase,the levels of FGF-9 and S100A9 in the serum of CHB patients showed a gradual upward trend(P<0.05).As the staging of liver fibrosis gradually increased,the levels of FGF-9 and S100A9 in the serum of CHB patients showed a gradually increasing trend(P<0.05).Pearson correlation analysis showed that there was a positive correlation between the levels of FGF-9,S100A9,and HBV-DNA in the serum of CHB patients(P<0.05).The levels of FGF-9,S100A9,and HBV-DNA in CHB patients were positively correlated with the degree of inflammation and liver fibrosis stage(P<0.05).FGF-9 levels and S100A9 water levels are independent risk factors for moderate to severe CHB(P<0.05).Conclusion FGF-9 and S100A9 in the serum of CHB pa-tients are correlated with the severity of the disease,and can be used as reference indicators to evaluate the severity of the disease in CHB patients.