不同碳源对耐热型解脂亚罗酵母生长的影响

Effect of different carbon sources on growth of thermoresistant Yarrowia lipolytica

【目的】探索不同碳源对耐热型解脂亚罗酵母生长及产赤藓糖醇的影响,通过优化碳源组合提升耐热型解脂亚罗酵母的赤藓糖醇产量。【方法】分别以30、34、38℃条件下生长的解脂亚罗酵母CA20、解脂亚罗酵母HT34和解脂亚罗酵母HT38为研究对象,利用含有不同碳源的培养基进行培养。培养过程中每隔24 h取样,采用酶标仪检测菌液在600 nm处的光密度值(optical density, OD_(600))。培养液中赤藓糖醇和葡萄糖质量浓度采用高效液相色谱(HPLC)检测。【结果】菌株CA20能利用葡萄糖、果糖、半乳糖、阿拉伯糖、木糖、麦芽糖、蔗糖、纤维二糖、甘露醇、山梨醇、甘油、可溶性淀粉和大豆油脂等碳源,其中葡萄糖、果糖、甘露醇、山梨醇、甘油和大豆油脂为最优碳源;菌株HT34对除葡萄糖和果糖以外碳源的利用能力下降,而菌株HT38几乎不能利用除葡萄糖和大豆油脂以外的碳源;培养基中同时添加葡萄糖和另一种碳源对菌株CA20生长的影响较小,而同时添加葡萄糖和大豆油脂可明显促进菌株HT34和HT38的生长。此外,大豆油脂可显著提升菌株HT34和HT38的赤藓糖醇产量和得率,增加量最高可达50%左右。【结论】本研究结果为今后利用高温发酵生产赤藓糖醇提供了理论依据。

[Objective]The effect of different carbon sources was investigated on the growth of thermoresistant Yarrowia lipolytica and erythritol synthesis to improve the production of erythritol from thermoresistant Yarrowia lipolytica by optimizing the combination of carbon sources.[Method]Culture media containing different carbon sources were used to cultivate the Y.lipolytica CA20,Y.lipolytica HT34,and Y.lipolytica HT38 grown at 30℃,34℃,and 38℃,respectively,during which the optical density of cell culture was detected at a wave length of 600 nm(OD_(600))with an interval of 24 h.Meanwhile,the concentrations of erythritol and glucose were assayed by using high performance liquid chromatography(HPLC).[Result]Strain CA20 is able to use such carbon sources as glucose,fructose,galactose,arabinose,xylose,maltose,sucrose,cellobiose,mannitol,sorbitol,glycerol,soluble starch,and soybean oil to grow,among which glucose,fructose,mannitol,glycerol,and soybean oil are the optimal carbon sources;the growth of HT34 is inhibited when using carbon sources except for glucose and fructose,while HT38 can only use glucose and soybean oil to support its growth;the addition of glucose and another one carbon source exerts little effect on the growth of strain CA20,while the addition of glucose and soybean oil promotes the growth of HT34 and HT38 obviously.In addition,the addition of soybean oil can significantly improve the erythritol production and yield by HT34 and HT38,with an increase as much as 50%.[Conclusion]These findings can provide a basis for the erythritol production using high temperature fermentation.