食管鳞癌组织中TIM-3、FOXA2表达与其临床病理特征及预后的关系研究

The Relationship Between the Expression of TIM-3 and FOXA2 in Esophageal Squamous Cell Carcinoma and Its Clinicopathological Features and Prognosis

目的探讨食管鳞癌组织中T细胞免疫球蛋白黏蛋白分子3(TIM-3)、叉头框蛋白A2(FOXA2)表达与其临床病理特征及预后的关系。方法选取87例食管鳞癌患者(食管鳞癌组)、50例慢性食管炎患者(对照组)为研究对象。分别比较食管鳞癌组与对照组、不同临床病理特征食管鳞癌患者及不同预后食管鳞癌患者TIM-3、FOXA2表达;采用Kaplan-Meier生存曲线进行生存分析,应用Cox比例风险回归模型分析TIM-3、FOXA2表达与食管鳞癌预后的关系。结果食管鳞癌组TIM-3阳性表达率高于对照组,FOXA2阳性表达率低于对照组(P<0.05);不同TNM分期、有无淋巴结转移及不同浸润深度的食管鳞癌患者TIM-3、FOXA2表达比较,差异有统计学意义(P<0.05);食管鳞癌组随访期间生存68例,死亡19例,死亡患者TIM-3阳性表达率高于生存患者,FOXA2阳性表达率低于生存患者(P<0.05);Kaplan-Meier生存曲线显示,TIM-3、FOXA2阴性表达患者总生存率较阳性表达患者高(P<0.05);Cox单因素回归分析显示,TNM分期、淋巴结转移、浸润深度、TIM-3、FOXA2是影响食管鳞癌患者预后的危险因素(P<0.05);Cox多因素分析显示,TNM分期、淋巴结转移、TIM-3、FOXA2是影响食管鳞癌患者预后的独立危险因素(P<0.05)。结论食管鳞癌组织中存在TIM-3、FOXA2表达异常,且与TNM分期、淋巴结转移、浸润深度及预后密切相关。

Objective To investigate the relationship between the expression of T-cell immunoglobulin and mucin domain containing molecule 3(TIM-3)and forkhead box A2(FOXA2)in esophageal squamous cell carcinoma and their clinicopathological characteristics and prognosis.Methods 87 patients with esophageal squamous cell carcinoma(esophageal squamous cell carcinoma group)and 50 patients with chronic Esophagitis(control group)were selected as the study subjects.Compared the expression of TIM-3 and FOXA2 between the esophageal squamous cell carcinoma group and the control group,esophageal squamous cell carcinoma patients with different clinical and pathological characteristics,and esophageal squamous cell carcinoma patients with different prognosis of esophageal squamous cell carcinoma.Kaplan-Meier survival curve was used for survival analysis,and Cox proportional hazard regression model was used to analyze the relationship between TIM-3,FOXA2 expression and prognosis of esophageal squamous cell carcinoma.Results The positive expression rate of TIM-3 in esophageal squamous cell carcinoma group was higher than that in control group,while the positive expression rate of FOXA2 in esophageal squamous cell carcinoma group was lower than that in control group(P<0.05).The expression of TIM-3 and FOXA2 in esophageal squamous cell carcinoma patients with different TNM stages,lymph node metastasis and different depth of invasion was statistically significant(P<0.05).In the esophageal squamous cell carcinoma group,68 patients survived and 19 died during the follow-up period.The positive expression rate of TIM-3 in the dead patients was higher than that in the living patients,while the positive expression rate of FOXA2 was lower than that in the living patients(P<0.05).Kaplan-Meier survival curve showed that the overall survival rate of patients with negative TIM-3 and FOXA2 expression was higher than that of patients with positive TIM-3 and FOXA2 expression(P<0.05).Cox single factor regression analysis showed that TNM stage,lymph node metastasis,depth of invasion,TIM-3,FOX-A2 were the risk factors affecting the prognosis of patients with esophageal squamous cell carcinoma(P<0.05).Cox multivariate analysis showed that TNM stage,lymph node metastasis,TIM-3 and FOXA2 were independent risk factors affecting the prognosis of patients with esophageal squamous cell carcinoma(P<0.05).Conclusion The abnormal expression of TIM-3 and FOXA2 in esophageal squamous cell carcinoma is closely related to TNM stage,lymph node metastasis,depth of invasion and prognosis.